Anti-Seladin 1 antibody [EPR26466-37]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] (AB314221)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Seladin 1 with ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse cardiac muscle. The section was incubated with ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] (AB314221)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Seladin 1 with ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Leydig cells of mouse testis. The section was incubated with ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] (AB314221)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Seladin 1 with ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] (AB314221)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Seladin 1 with ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Seladin 1 antibody [EPR26466-37] (AB314221)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Performed under reducing conditions. In Western blot, ab314221 was shown to bind specifically to Seladin 1. Target of interest was observed at 54 kDa in wild-type A431 cell lysates (lane 2) with no signal observed at this size in DHCR24 knockout cell line (lane 3) (lane 3, knockout cell line ab262480 / knockout cell lysate ab263920). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1-5 : 10 seconds, Lane 6-8 : 125 seconds, Lane 9 : 180 seconds.

All lanes:

Western blot - Anti-Seladin 1 antibody [EPR26466-37] (ab314221) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Wild-type A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

DHCR24 KO A431 whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Lane 7:

Mouse lung tissue lysate at 20 µg

Lane 8:

Rat testis tissue lysate at 20 µg

Lane 9:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 54 kDa

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