Knockout Tested Rabbit Recombinant Monoclonal Seladin 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421119.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421118.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421117.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421116.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222)")
![Western blot - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--western-blot-img421040.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (AB314222)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis (PubMed:26114596). In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis (By similarity).
Alternative names
Kiaa0018, Dhcr24, Delta(24)-sterol reductase, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal Seladin 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26466-37
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human and rat IHC-P.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314222 is the carrier-free version of Anti-Seladin 1 antibody [EPR26466-37] ab314221.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Seladin 1 also known as DHCR24 (3β-Hydroxysteroid-Δ24 reductase) is an enzyme with a molecular mass of approximately 54 kDa. It plays a role in cholesterol biosynthesis by catalyzing the reduction of a double bond at the C-24 position in sterol precursors. Seladin 1 is expressed in various tissues with high expression in the brain liver and adrenal gland. The enzyme contributes to the maintenance of cellular cholesterol homeostasis and provides neuroprotective functions.
- Biological function summary
Seladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.
- Pathways
Seladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.
- Associated diseases and disorders
Seladin 1 has been implicated in Alzheimer's disease and certain cancers. In Alzheimer's disease a reduction in Seladin 1 expression correlates with increased susceptibility to amyloid-beta toxicity while its overexpression shows a protective effect. This relationship ties Seladin 1 to amyloid precursor protein processing. In some cancers altered Seladin 1 expression affects tumor growth and progression where it may interact with proteins involved in cell survival pathways. This dual role in both neurodegenerative and cancerous conditions highlights the importance of Seladin 1 in human health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421119.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)
This data was developed using Anti-Seladin 1 antibody [EPR26466-37] ab314221, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Seladin 1 with Anti-Seladin 1 antibody [EPR26466-37] ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cardiac muscle. The section was incubated with Anti-Seladin 1 antibody [EPR26466-37] ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421118.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)
This data was developed using Anti-Seladin 1 antibody [EPR26466-37] ab314221, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Seladin 1 with Anti-Seladin 1 antibody [EPR26466-37] ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Leydig cells of mouse testis. The section was incubated with Anti-Seladin 1 antibody [EPR26466-37] ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421117.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)
This data was developed using Anti-Seladin 1 antibody [EPR26466-37] ab314221, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Seladin 1 with Anti-Seladin 1 antibody [EPR26466-37] ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with Anti-Seladin 1 antibody [EPR26466-37] ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421116.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)
This data was developed using Anti-Seladin 1 antibody [EPR26466-37] ab314221, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Seladin 1 with Anti-Seladin 1 antibody [EPR26466-37] ab314221 at 1/100 (5.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with Anti-Seladin 1 antibody [EPR26466-37] ab314221 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Western blot - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222), expandable thumbnail](https://content.abcam.com/products/images/seladin-1-antibody-epr26466-37-bsa-and-azide-free-ab314222--western-blot-img421040.jpg "Western blot - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)")
Western blot - Anti-Seladin 1 antibody [EPR26466-37] - BSA and Azide free (ab314222)
This data was developed using Anti-Seladin 1 antibody [EPR26466-37] ab314221, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Performed under reducing conditions.
In Western blot, Anti-Seladin 1 antibody [EPR26466-37] ab314221 was shown to bind specifically to Seladin 1. Target of interest was observed at 54 kDa in wild-type A431 cell lysates (lane 2) with no signal observed at this size in DHCR24 knockout cell line (lane 3) (lane 3, knockout cell line Human DHCR24 knockout A-431 cell line ab262480 / knockout cell lysate Human DHCR24 knockout A-431 cell lysate ab263920).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-5: 10 seconds, Lane 6-8: 125 seconds, Lane 9: 180 seconds.All lanes: Western blot - Anti-Seladin 1 antibody [EPR26466-37] (Anti-Seladin 1 antibody [EPR26466-37] ab314221) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Wild-type A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: DHCR24 KO A431 whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: Mouse liver tissue lysate at 20 µg
Lane 6: Mouse testis tissue lysate at 20 µg
Lane 7: Mouse lung tissue lysate at 20 µg
Lane 8: Rat testis tissue lysate at 20 µg
Lane 9: Human lung tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Performed under reducing conditions.
Observed band size: 54 kDa
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