Rabbit Polyclonal Selenium Binding Protein 1/SBP antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 7 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IP | WB | IHC-P | ICC/IF | |
---|---|---|---|---|
Human | Expected | Tested | Tested | Tested |
Mouse | Tested | Tested | Expected | Expected |
Rat | Expected | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria (PubMed:29255262). Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport (By similarity).
SBP, SBP, SELENBP1, Methanethiol oxidase, MTO, 56 kDa selenium-binding protein, Selenium-binding protein 1, SBP56, SP56
Rabbit Polyclonal Selenium Binding Protein 1/SBP antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 7 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Selenium Binding Protein 1 (SBP1) also known as SELENBP1 is a protein of molecular weight approximately 56 kDa. It is involved in various cellular mechanisms. SBP1 binds selenium thereby playing a role in selenium metabolism and regulation within the cell. It is expressed in numerous tissues including liver kidney and colon indicating a broad physiological significance. Its expression is subject to tight regulation pointing to its importance in cellular functions.
Selenium Binding Protein 1 interacts with selenium implicating it in various metabolic processes. It does not act as part of a larger complex but functions independently to influence cellular responses to selenium levels. Research shows that SBP1 plays a role in antioxidant defense and cellular detoxification suggesting a protective function against oxidative stress. This regulation supports various metabolic pathways by modulating selenium-dependent enzyme activity.
Selenium Binding Protein 1 has been connected to the antioxidant defense pathway and the detoxification process. It interacts with proteins involved in these pathways such as glutathione peroxidases essential for eliminating oxidative damage in cells. By influencing these pathways SBP1 helps maintain cellular homeostasis highlighting its role in the broader metabolic network.
Aberrations in Selenium Binding Protein 1 levels are linked to certain cancers and neurological disorders. Studies show a decrease in SBP1 expression in cancers such as colorectal cancer possibly related to reduced cellular detoxification capacity. Additionally some neurological disorders have shown altered SBP1 expression which could connect the protein with enhanced oxidative stress and cell damage. By affecting conditions like cancer SBP1 could interact with proteins involved in cell proliferation and survival supporting research on therapeutic targets.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Lanes 1-3: Merged signal (red and green). Green - ab90135 observed at 60 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab90135 Anti-Selenium Binding Protein 1/SBP antibody was shown to specifically react with Selenium Binding Protein 1/SBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line ab265279 (knockout cell lysate Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate ab257662) was used. Wild-type and Selenium Binding Protein 1/SBP knockout samples were subjected to SDS-PAGE. ab90135 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SELENBP1 knockout HeLa cell lysate at 20 µg
Lane 3: HT-29 cell lysate at 20 µg
Lane 4: SW 480 cell lysate at 20 µg
Lanes 1 - 4: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Lane 4: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa
All lanes: Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/mL
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 2: Human colon tissue lysate - total protein (ab30051) at 10 µg
Lane 3: Human spleen tissue lysate - total protein (ab29699) at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 50 kDa, 52 kDa
Exposure time: 1min
ICC/IF image of ab90135 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml.
IHC image of ab90135 staining in Normal Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90135, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Selenium Binding Protein 1/SBP was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5μg of Rabbit polyclonal to Selenium Binding Protein 1/SBP and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90135.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 52kDa; Selenium Binding Protein 1/SBP
All lanes: Immunoprecipitation - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
Predicted band size: 52 kDa
All lanes: Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/mL
Lane 1: Liver (Mouse) Tissue Lysate at 10 µg
Lane 2: Lung (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 1min
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