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AB153923

Anti-Selenophosphate synthetase 1 antibody

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(1 Publication)

Rabbit Polyclonal Selenophosphate synthetase 1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPHS1 aa 150 to C-terminus.

View Alternative Names

SELD, SPS, SPS1, SEPHS1, Zincore component SEPHS1, Selenium donor protein 1, Selenophosphate synthase 1

1 Images
Western blot - Anti-Selenophosphate synthetase 1 antibody (AB153923)
  • WB

Unknown

Western blot - Anti-Selenophosphate synthetase 1 antibody (AB153923)

10% SDS PAGE

All lanes:

Western blot - Anti-Selenophosphate synthetase 1 antibody (ab153923) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SEPHS1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49903

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Selenophosphate synthetase 1 also known as SEPHS1 or SPS1 catalyzes the synthesis of selenophosphate from selenide and ATP. It plays a critical role in the production of selenium-containing biomolecules. The mass of SEPHS1 approximates 46 kDa. SEPHS1 is expressed in various tissues including the liver and kidneys suggesting its involvement across multiple biological processes. Researchers often recognize SEPHS1 for its activity in selenium metabolism.
Biological function summary

The protein is involved in the synthesis of selenoproteins important for cellular antioxidant defense and redox homeostasis. Selenophosphate synthetase 1 functions as part of a multi-enzyme complex essential for the biosynthesis of selenocysteine the 21st amino acid which is then incorporated into selenoproteins. These selenoproteins include glutathione peroxidases and thioredoxin reductases which are important for maintaining cellular integrity.

Pathways

The protein participates actively in the selenocysteine biosynthesis and recycling pathway ensuring adequate supply of selenophosphate needed for selenoprotein production. Within this pathway SEPHS1 closely associates with several enzymes like selenocysteine synthase and selenocysteine tRNA (tRNASec) which facilitate the incorporation of selenocysteine into proteins. These interactions highlight the interconnected role of SEPHS1 in maintaining proper cellular functions through selenoproteins.

SEPHS1 is linked to deficiency-related conditions such as Keshan disease a type of heart disorder due to low selenium levels and certain thyroid dysfunctions linked to disrupted selenoprotein synthesis. Disruption of SEPHS1 function impairs selenoprotein production impacting proteins like glutathione peroxidase and thioredoxin reductase which are vital in preventing oxidative damage hence making SEPHS1 significant in understanding and potentially mitigating such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the zincore complex, a heterotetramer that acts as a molecular 'grip' to stabilize transcription factors at DNA-binding sites across the genome, thereby controlling gene expression (PubMed : 40608935). The zincore complex binds specifically to zinc finger transcription factors, such as ZFP91, ZNF652, ZNF526 and PRDM15, and stabilizes them onto their cognate DNA motif (PubMed : 40608935). Within the complex, SEPHS1, recognizes and binds the backbone of zinc fingers of transcription factors in a sequence-independent manner via its arginine clamp, enhancing their DNA-binding stability (PubMed : 40608935). Plays an essential role in redox homeostasis (PubMed : 31607477). May also be involved in selenocysteine biosynthesis by catalyzing formation of selenophosphate from selenide and ATP (PubMed : 7665581). Its role in selenocysteine biosynthesis is however unclear and several studies suggest that it does not act as a selenophosphate synthase in vivo or plays an non-essential role (PubMed : 15534230). Required for cardiac differentiation (By similarity).
See full target information SEPHS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Military Medical Research 9:4 PubMed35057868

2022

Potential roles of vitamin D binding protein in attenuating liver injury in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xiao,Du-Chao Zhang,Ye Hu,Li-Cheng Song,Jian-Qiao Xu,Wan-Xue He,Pan Pan,Yu-Wei Wang,Li-Xin Xie
View all publications
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Product promise

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