Rabbit Polyclonal Selenophosphate synthetase 1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPHS1 aa 1-200.
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- Nature communications 10:47102019Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Akieda et. al.PubMed 31624259
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SEPHS1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49903
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Synthesizes selenophosphate from selenide and ATP.
Alternative names
SELD, SPS, SPS1, SEPHS1, Selenium donor protein 1, Selenophosphate synthase 1
Recommended products
Rabbit Polyclonal Selenophosphate synthetase 1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPHS1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SEPHS1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49903
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Selenophosphate synthetase 1 also known as SEPHS1 or SPS1 catalyzes the synthesis of selenophosphate from selenide and ATP. It plays a critical role in the production of selenium-containing biomolecules. The mass of SEPHS1 approximates 46 kDa. SEPHS1 is expressed in various tissues including the liver and kidneys suggesting its involvement across multiple biological processes. Researchers often recognize SEPHS1 for its activity in selenium metabolism.
- Biological function summary
The protein is involved in the synthesis of selenoproteins important for cellular antioxidant defense and redox homeostasis. Selenophosphate synthetase 1 functions as part of a multi-enzyme complex essential for the biosynthesis of selenocysteine the 21st amino acid which is then incorporated into selenoproteins. These selenoproteins include glutathione peroxidases and thioredoxin reductases which are important for maintaining cellular integrity.
- Pathways
The protein participates actively in the selenocysteine biosynthesis and recycling pathway ensuring adequate supply of selenophosphate needed for selenoprotein production. Within this pathway SEPHS1 closely associates with several enzymes like selenocysteine synthase and selenocysteine tRNA (tRNASec) which facilitate the incorporation of selenocysteine into proteins. These interactions highlight the interconnected role of SEPHS1 in maintaining proper cellular functions through selenoproteins.
- Associated diseases and disorders
SEPHS1 is linked to deficiency-related conditions such as Keshan disease a type of heart disorder due to low selenium levels and certain thyroid dysfunctions linked to disrupted selenoprotein synthesis. Disruption of SEPHS1 function impairs selenoprotein production impacting proteins like glutathione peroxidase and thioredoxin reductase which are vital in preventing oxidative damage hence making SEPHS1 significant in understanding and potentially mitigating such disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 1 antibody (ab96542)
ab96542 at 1/200 dilution staining Selenophosphate synthetase 1 in paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
Western blot - Anti-Selenophosphate synthetase 1 antibody (ab96542)
12% SDS PAGE
All lanes: Western blot - Anti-Selenophosphate synthetase 1 antibody (ab96542) at 1/500 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: Molt4 whole cell lysate at 30 µg
Predicted band size: 43 kDa
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