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AB153878

Anti-Selenophosphate synthetase 2 antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal Selenophosphate synthetase 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEPHS2 aa 100 to C-terminus.

View Alternative Names

SPS2, SEPHS2, Selenium donor protein 2, Selenophosphate synthase 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (AB153878)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (AB153878)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells, labeling Selenophosphate synthetase 2 with ab153878 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenophosphate synthetase 2 antibody (AB153878)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenophosphate synthetase 2 antibody (AB153878)

Immunohistochemical analysis of paraffin-embedded Human OVCA tissue, labeling Selenophosphate synthetase 2 with ab153878 at 1/100 dilution

Western blot - Anti-Selenophosphate synthetase 2 antibody (AB153878)
  • WB

Unknown

Western blot - Anti-Selenophosphate synthetase 2 antibody (AB153878)

10% SDS PAGE

All lanes:

Western blot - Anti-Selenophosphate synthetase 2 antibody (ab153878) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SEPHS2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99611

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Alternative Products

Primary Antibodies

AB96541

Anti-Selenophosphate synthetase 2 antibody

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Complementary Products

Primary Antibodies

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Anti-Selenophosphate synthetase 1 antibody

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Primary Antibodies

AB194554

Anti-SelI antibody

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Primary Antibodies

AB124954

Anti-TXNRD1 antibody [EPNCIR129]

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Primary Antibodies

AB248613

Anti-SelM antibody [EPR8279] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SelM antibody [EPR8279] - BSA and Azide free (AB248613)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Selenophosphate synthetase 2 (SPS2) also known as SEPHS2 plays a pivotal role in selenium metabolism by synthesizing selenophosphate from selenide and ATP. This reaction is vital for the synthesis of selenocysteine the 21st amino acid incorporated into various selenoproteins. SPS2 is a 47 kDa protein expressed broadly with notable presence in tissues such as liver kidney and brain reflecting the need for selenium in these organs. Its activity aligns with the body's demand for selenium an important dietary trace element involved in various biological processes.
Biological function summary

SPS2 serves as an important enzyme in the selenoprotein biosynthesis pathway enabling the construction of selenophosphate which is then utilized for selenocysteine formation in selenoproteins. These proteins are integral to oxidative stress defense and redox homeostasis. While SPS2 itself is not part of a larger protein complex its function strongly influences the activity and formation of several selenoproteins each requiring selenium for their catalytic efficiency and structural integrity.

Pathways

SPS2 is critical for the selenocysteine biosynthesis pathway. This pathway directly impacts protein translation mechanisms that incorporate selenocysteine into selenoproteins distinguishing selenoproteins from other proteins. SPS2 is involved in this pathway alongside other key proteins such as selenocysteine synthase and selenocysteine-specific elongation factor. SPS2's role in this pathway highlights its importance in maintaining cellular redox states and combating oxidative stress.

SPS2 demonstrates relevance to disorders like Kashin-Beck disease and certain types of cancer where selenium metabolism imbalances are implicated. The protein's function affects the stability and synthesis of selenoproteins like glutathione peroxidase and thioredoxin reductase which are known for their role in antioxidative defenses. Any disruption in SPS2 expression or function can lead to oxidative stress-related tissue damage highlighting its significance in maintaining cellular health and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Synthesizes selenophosphate from selenide and ATP.
See full target information SEPHS2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 2:100360 PubMed34467244

2021

Secreted retrovirus-like GAG-domain-containing protein PEG10 is regulated by UBE3A and is involved in Angelman syndrome pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Nikhil J Pandya,Congwei Wang,Veronica Costa,Paul Lopatta,Sonja Meier,F Isabella Zampeta,A Mattijs Punt,Edwin Mientjes,Philip Grossen,Tania Distler,Manuel Tzouros,Yasmina Martí,Balazs Banfai,Christoph Patsch,Soren Rasmussen,Marius Hoener,Marco Berrera,Thomas Kremer,Tom Dunkley,Martin Ebeling,Ben Distel,Ype Elgersma,Ravi Jagasia

Frontiers in nutrition 8:665855 PubMed34381803

2021

Effects of Selenomethionine on Cell Viability, Selenoprotein Expression and Antioxidant Function in Porcine Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Chen,Yinzhi Zhang,Yantao Lv,Min Tian,Jinming You,Fang Chen,Shihai Zhang,Wutai Guan
View all publications
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Product promise

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