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AB96541

Anti-Selenophosphate synthetase 2 antibody

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(2 Publications)

Rabbit Polyclonal Selenophosphate synthetase 2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEPHS2 aa 150 to C-terminus.

View Alternative Names

SPS2, SEPHS2, Selenium donor protein 2, Selenophosphate synthase 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (AB96541)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (AB96541)

ab96541, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence (Panel

  1. and merged with DNA probe (Panel 2).
Western blot - Anti-Selenophosphate synthetase 2 antibody (AB96541)
  • WB

Unknown

Western blot - Anti-Selenophosphate synthetase 2 antibody (AB96541)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Selenophosphate synthetase 2 antibody (ab96541) at 1/1000 dilution

All lanes:

Hela S3 whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SEPHS2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99611

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Alternative Products

Primary Antibodies

AB153878

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB248613

Anti-SelM antibody [EPR8279] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SelM antibody [EPR8279] - BSA and Azide free (AB248613)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Selenophosphate synthetase 2 (SPS2) also known as SEPHS2 plays a pivotal role in selenium metabolism by synthesizing selenophosphate from selenide and ATP. This reaction is vital for the synthesis of selenocysteine the 21st amino acid incorporated into various selenoproteins. SPS2 is a 47 kDa protein expressed broadly with notable presence in tissues such as liver kidney and brain reflecting the need for selenium in these organs. Its activity aligns with the body's demand for selenium an important dietary trace element involved in various biological processes.
Biological function summary

SPS2 serves as an important enzyme in the selenoprotein biosynthesis pathway enabling the construction of selenophosphate which is then utilized for selenocysteine formation in selenoproteins. These proteins are integral to oxidative stress defense and redox homeostasis. While SPS2 itself is not part of a larger protein complex its function strongly influences the activity and formation of several selenoproteins each requiring selenium for their catalytic efficiency and structural integrity.

Pathways

SPS2 is critical for the selenocysteine biosynthesis pathway. This pathway directly impacts protein translation mechanisms that incorporate selenocysteine into selenoproteins distinguishing selenoproteins from other proteins. SPS2 is involved in this pathway alongside other key proteins such as selenocysteine synthase and selenocysteine-specific elongation factor. SPS2's role in this pathway highlights its importance in maintaining cellular redox states and combating oxidative stress.

SPS2 demonstrates relevance to disorders like Kashin-Beck disease and certain types of cancer where selenium metabolism imbalances are implicated. The protein's function affects the stability and synthesis of selenoproteins like glutathione peroxidase and thioredoxin reductase which are known for their role in antioxidative defenses. Any disruption in SPS2 expression or function can lead to oxidative stress-related tissue damage highlighting its significance in maintaining cellular health and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Synthesizes selenophosphate from selenide and ATP.
See full target information SEPHS2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8030 PubMed40877267

2025

Organic-inorganic covalent selenium reversing ischemic reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zushuang Xiong,Guanning Huang,Jia-Run Huang,Ying Liu,Lizhen He,Tianfeng Chen

Acta neuropathologica communications 9:23 PubMed33557929

2021

A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Yuan Qian,Yu-Dong Guo,Juan Zu,Jin-Hua Zhang,Yi-Ming Zheng,Idriss Ali Abdoulaye,Zhao-Hui Pan,Chun-Ming Xie,Han-Chao Gao,Zhi-Jun Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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