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AB194554

Anti-SelI antibody

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(4 Publications)

Mouse Polyclonal SelI antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SELENOI aa 1-50.

View Alternative Names

EPT1, KIAA1724, SELI, SELENOI, Ethanolaminephosphotransferase 1, hEPT1, Selenoprotein I, SelI

2 Images
Western blot - Anti-SelI antibody (AB194554)
  • WB

Supplier Data

Western blot - Anti-SelI antibody (AB194554)

All lanes:

Western blot - Anti-SelI antibody (ab194554) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 45 kDa

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Western blot - Anti-SelI antibody (AB194554)
  • WB

Supplier Data

Western blot - Anti-SelI antibody (AB194554)

All lanes:

Western blot - Anti-SelI antibody (ab194554) at 1/1000 dilution

All lanes:

recombinant fragment of Human SelI protein (the immunogen) at 0.2 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SELENOI aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9C0D9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p>Use at 1/1500 - 1/1000 dilution for cell lysates and at 1/1000 - 1/2500 for recombinant protein.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p>Use at 1/1500 - 1/1000 dilution for cell lysates and at 1/1000 - 1/2500 for recombinant protein.</p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SelI also known as selenoprotein I plays an important role in synthesizing selenocysteine a rare amino acid with antioxidant functions. It has a molecular mass of around 65 kDa. SelI is expressed in a wide range of tissues with notable levels detected in the liver brain and heart. This distribution suggests its involvement in several essential cellular processes across different organ systems.
Biological function summary

SelI contributes by supporting selenoprotein biosynthesis and function underpinning cellular antioxidative processes and redox homeostasis. As a part of a larger multi-protein complex SelI ensures the efficient incorporation of selenocysteine into proteins which is essential for the function of many enzymes. Its integration into these complexes highlights its importance in maintaining cellular health and preventing oxidative damage.

Pathways

SelI stands out in the selenoprotein synthesis pathway. This pathway involves the interplay of other proteins including SECIS-binding protein 2 (SBP2) and selenophosphate synthetase 2. It also intersects with the glutathione metabolic pathway linking SelI to cellular detoxification systems. These connections allow SelI to play a part in controlling oxidative stress through well-known antioxidant mechanisms.

SelI has significant connections to neurodegenerative diseases such as Alzheimer's disease and liver disorders. Its relationship with Alzheimer’s is through its role in managing oxidative stress which has implications in the neurodegeneration seen in this disorder. Meanwhile in liver disorders SelI's function ties to its selenoprotein-related antioxidative capabilities working with proteins like glutathione peroxidase to guard against liver damage. These associations mark SelI as an important focus of study in understanding and potentially managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ethanolaminephosphotransferase that catalyzes the transfer of phosphoethanolamine (PE) from CDP-ethanolamine to lipid acceptors, the final step in the synthesis of PE via the 'Kennedy' pathway (PubMed : 17132865, PubMed : 28052917, PubMed : 29500230). PE is the second most abundant phospholipid of membranes in mammals and is involved in various membrane-related cellular processes (PubMed : 17132865). The enzyme is critical for the synthesis of several PE species and also catalyzes the synthesis of plasmanyl-PE, a lipid required for proper myelination and neurodevelopment, from 1-alkyl-2-acylglycerol (PubMed : 29500230).
See full target information SELENOI
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404073 PubMed38757622

2024

SELENOI Functions as a Key Modulator of Ferroptosis Pathway in Colitis and Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Huang,Xu Yang,Mingxin Zhang,Tong Li,Kongdi Zhu,Yulan Dong,Xingen Lei,Zhengquan Yu,Cong Lv,Jiaqiang Huang

Journal of pharmaceutical analysis 13:1281-1295 PubMed38174118

2024

Oridonin restores hepatic lipid homeostasis in an LXRα-ATGL/EPT1 axis-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Chen,Huanguo Jiang,Zhikun Zhan,Jindi Lu,Tanwei Gu,Ping Yu,Weimin Liang,Xi Zhang,Shilong Zhong,Lan Tang

Cell death & disease 14:85 PubMed36746922

2023

Restoration of lipid homeostasis between TG and PE by the LXRα-ATGL/EPT1 axis ameliorates hepatosteatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Chen,Huanguo Jiang,Zhikun Zhan,Jindi Lu,Tanwei Gu,Ping Yu,Weimin Liang,Xi Zhang,Shuwen Liu,Huichang Bi,Shilong Zhong,Lan Tang

The journal of obstetrics and gynaecology research 43:1180-1188 PubMed28544021

2017

Differential expression of EWI-2 in endometriosis, its functional role and underlying molecular mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Zheng,Jing Yang
View all publications
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Product promise

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