AB121276

Anti-SELK antibody

Name: Anti-SELK antibody (ab121276) | Abcam
Brand: Abcam Limited
SKU: AB121276
Price: 645 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SELK antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SELENOK aa 1 to C-terminus.

View Alternative Names

SELK, HSPC030, HSPC297, SELENOK, Selenoprotein K, SelK

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human testis tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human liver tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Supplier Data

Immunocytochemistry - Anti-SELK antibody (AB121276)

Immunofluorescent staining of U-251 MG (Human brain glioma) cells labeling SELK with ab121276 at 0.25 ug/ml (green).

Supplier Data

Western blot - Anti-SELK antibody (AB121276)

Western blot analysis of HepG2 cells labeling SELK with ab121276 at 0.04 ug/ml.

All lanes:

Western blot - Anti-SELK antibody (ab121276) at 0.04 µg/mL

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human SELENOK aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6D0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SELK also known as selenoprotein K plays an important role in cellular processes. SELK is a small protein with a mass of about 11.4 kDa. It is characterized by the presence of the amino acid selenocysteine which is rare and important for its function. SELK produces in various tissues including heart skeletal muscle and liver. It locates in the endoplasmic reticulum membrane where it interacts with other proteins to contribute to molecular processes essential for cell homeostasis.

Biological function summary

SELK influences the regulation of calcium flux in cells affecting cellular signaling and immune responses. It is not a stand-alone actor but associates with other proteins to form multi-protein complexes that facilitate these cellular functions. SELK participates in processes like antioxidant defense and endoplasmic reticulum stress response which protects cells from damage. By modulating the immune response SELK plays an important role in maintaining balance in the immune system.

Pathways

This protein interacts in the oxidative stress pathways and plays a role in calcium signaling. In oxidative stress pathways SELK helps protect cells against reactive oxygen species maintaining cellular health. It works closely with proteins such as selenoprotein S and other antioxidant defense proteins. In calcium signaling SELK helps regulate calcium ion flow across cellular membranes supporting various cellular functions essential for those pathways.

SELK connects to cardiovascular diseases and autoimmune disorders. Its involvement in regulating immune responses and calcium flux associates with the pathogenesis of these conditions. In cardiovascular diseases abnormal SELK expression may contribute to heart abnormalities by interacting with calcium-modulating proteins like calnexin. In autoimmune disorders a dysregulated SELK function may affect immune balance and lead to immune system malfunction. Understanding SELK interactions gives insights into these diseases and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for Ca(2+) flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration (By similarity). Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins (PubMed : 22016385). Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation (By similarity). Together with ZDHHC6, required for palmitoylation of ITPR1 in immune cells, leading to regulate ITPR1 stability and function (PubMed : 25368151). Plays a role in protection of cells from ER stress-induced apoptosis (PubMed : 20692228). Protects cells from oxidative stress when overexpressed in cardiomyocytes (PubMed : 16962588).

See full target information SELENOK

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8030 PubMed40877267

2025

Organic-inorganic covalent selenium reversing ischemic reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zushuang Xiong,Guanning Huang,Jia-Run Huang,Ying Liu,Lizhen He,Tianfeng Chen

Cell and tissue research 401:59-68 PubMed40323407

2025

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Steingruber,Simona Handtke,Franziska Schweiger,Stefan Schiele,Bruno Märkl,Marco Koch

International journal of nanomedicine 19:2071-2090 PubMed38476275

2024

MSC-Derived Exosomes Mitigate Myocardial Ischemia/Reperfusion Injury by Reducing Neutrophil Infiltration and the Formation of Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Feng,Xue Bao,Jinxuan Zhao,Lina Kang,Xuan Sun,Biao Xu

Redox biology 57:102500 PubMed36252341

2022

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue You,Fan Wu,Meilin Gao,Mengyue Chen,Shu Zeng,Yang Zhang,Wei Zhao,Danyang Li,Li Wei,Xiong Z Ruan,Yaxi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SELK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (AB121276)

Immunocytochemistry - Anti-SELK antibody (AB121276)

Western blot - Anti-SELK antibody (AB121276)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise