AB133681

Anti-SelM antibody [EPR8279]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal SELM antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

SELM, SELENOM, Selenoprotein M, SelM

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SelM antibody [EPR8279] (AB133681)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SelM antibody [EPR8279] (AB133681)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SelM with ab133681 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SelM antibody [EPR8279] (AB133681)

All lanes:

Western blot - Anti-SelM antibody [EPR8279] (ab133681) at 1/1000 dilution

Lane 1:

Human ovary cancer lysate at 10 µg

Lane 2:

Human stomach lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 16 kDa

false

Carrier free

Anti-SelM antibody [EPR8279] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8279

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SelM also known as selenoprotein M weighs approximately 14 kDa. SelM contains selenocysteine an amino acid incorporating selenium and is structurally small. Researchers identified SelM expression in various tissues including the brain liver and lungs. The exact cellular localization can vary according to the tissue type and the physiological conditions. SelM belongs to the family of selenoproteins which play numerous roles in redox homeostasis and enzyme regulation.

Biological function summary

SelM contributes significantly to intracellular antioxidant defense processes. It plays a role in maintaining the balance of reactive oxygen species (ROS) and protects cells from oxidative stress. Some findings suggest the association of SelM with protein complexes that combat oxidative damage although the specific interacting partners need further research. The protective function helps stabilize the cellular environment in challenging oxidative conditions.

Pathways

The selenoprotein SelM participates in antioxidant pathways such as the glutathione metabolic pathway. SelM may interact with other selenoproteins like GPx1 a glutathione peroxidase to help detoxify harmful peroxides. Furthermore SelM can be an integral component of cellular defense systems that maintain cell viability by controlling ROS levels and promoting a balanced redox state within cells.

SelM's role in oxidative stress management links it to neurodegenerative diseases such as Alzheimer's disease. Oxidative stress is a known factor in these diseases and SelM’s antioxidant function suggests potential protective effects. Evidence although limited implies a connection between SelM and proteins like amyloid precursor protein (APP) which could influence its pathogenicity in Alzheimer’s. Additionally disruptions in SelM expression or function might connect to metabolic disorders that involve oxidative damage. Further studies should clarify these relationships and ascertain SelM's therapeutic relevance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as a thiol-disulfide oxidoreductase that participates in disulfide bond formation.

See full target information SELENOM

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34298926

2021

Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan Sonet,Anne-Laure Bulteau,Zahia Touat-Hamici,Maurine Mosca,Katarzyna Bierla,Sandra Mounicou,Ryszard Lobinski,Laurent Chavatte

Antioxidants (Basel, Switzerland) 10: PubMed33805536

2021

Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish .

Applications

Unspecified application

Species

Unspecified reactive species

Dian-Guang Zhang,Tao Zhao,Xiao-Jian Xu,Wu-Hong Lv,Zhi Luo

Biochimica et biophysica acta. General subjects 1862:2493-2505 PubMed29660373

2018

Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.

Applications

Unspecified application

Species

Human

Zahia Touat-Hamici,Anne-Laure Bulteau,Juliusz Bianga,Hélène Jean-Jacques,Joanna Szpunar,Ryszard Lobinski,Laurent Chavatte

The Prostate 78:279-288 PubMed29314169

2018

Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dede N Ekoue,Emmanuel Ansong,Li Liu,Virgilia Macias,Ryan Deaton,Craig Lacher,Matthew Picklo,Larisa Nonn,Peter H Gann,Andre Kajdacsy-Balla,Gail S Prins,Vincent L Freeman,Alan M Diamond

The Journal of biological chemistry 289:6299-310 PubMed24425862

2014

Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts.

Applications

Species

Human

Yona Legrain,Zahia Touat-Hamici,Laurent Chavatte

Carcinogenesis 33:1225-30 PubMed22436612

2012

Selenoproteins reduce susceptibility to DMBA-induced mammary carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tamaro S Hudson,Bradley A Carlson,Mark J Hoeneroff,Heather A Young,Lorraine Sordillo,William J Muller,Dolph L Hatfield,Jeffrey E Green

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SelM antibody [EPR8279]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SelM antibody [EPR8279] (AB133681)

Western blot - Anti-SelM antibody [EPR8279] (AB133681)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise