AB190247

Anti-SELS antibody

Name: Anti-SELS antibody (ab190247) | Abcam
Brand: Abcam Limited
SKU: AB190247
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SELS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SELENOS aa 100 to C-terminus.

View Alternative Names

SELS, VIMP, AD-015, SBBI8, SELENOS, Selenoprotein S, SelS, VCP-interacting membrane protein

2 Images

Supplier Data

Immunoprecipitation - Anti-SELS antibody (AB190247)

Immunoprecipitation analysis of HeLa cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling SELS using ab190247 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated SELS, ab190247 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-SELS antibody (ab190247)

Predicted band size: 21 kDa

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Supplier Data

Western blot - Anti-SELS antibody (AB190247)

All lanes:

Western blot - Anti-SELS antibody (ab190247) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate in NETN buffer at 50 µg

Lane 2:

HeLa whole cell lysate in RIPA buffer at 50 µg

Lane 3:

293T whole cell lysate in NETN buffer at 50 µg

Lane 4:

Jurkat whole cell lysate in RIPA buffer at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SELENOS aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BQE4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab190247 was affinity purified using an epitope specific to SELS immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Selenoprotein S (SELS) also known as VIMP has a significant role in promoting the degradation of misfolded proteins in the endoplasmic reticulum (ER). It is part of the selenoprotein family incorporating selenium in the form of selenocysteine. The protein has a molecular mass of approximately 22 kDa. SELS localizes to the membrane of the ER and is expressed broadly across various tissues showing higher levels in liver and muscle.

Biological function summary

SELS participates actively in the regulation of ER stress response and inflammation. It functions as an important component of the ER-associated degradation (ERAD) pathway mediating the extraction and degradation of faulty proteins. This activity stems from its ability to interact with ubiquitin ligase complexes aiding in protein quality control. Its role is integral for maintaining cellular homeostasis and preventing accumulation of protein aggregates.

Pathways

SELS engages profoundly with the oxidative stress and inflammatory pathways. It modulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling pathway which influences inflammation and immune responses. SELS also interfaces with other proteins like p97/VCP and Derlin-1 which assist in the ERAD process demonstrating its connectivity within cellular stress response pathways.

SELS has connections to metabolic syndrome and inflammatory diseases. Altered expression levels of SELS are linked to the development of type 2 diabetes and obesity by influencing inflammatory mechanisms. SELS interacts with inflammatory cytokines like tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) highlighting its relevance in modulating immune responses linked to these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.

See full target information SELENOS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8030 PubMed40877267

2025

Organic-inorganic covalent selenium reversing ischemic reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zushuang Xiong,Guanning Huang,Jia-Run Huang,Ying Liu,Lizhen He,Tianfeng Chen

Nature communications 11:1769 PubMed32286295

2020

Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Ma,Keke Wei,Junwei Liu,Ke Tang,Huafeng Zhang,Liyan Zhu,Jie Chen,Fei Li,Pingwei Xu,Jie Chen,Jincheng Liu,Haiqing Fang,Liang Tang,Dianheng Wang,Liping Zeng,Weiwei Sun,Jing Xie,Yuying Liu,Bo Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SELS antibody

Immunoprecipitation - Anti-SELS antibody (AB190247)

Western blot - Anti-SELS antibody (AB190247)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise