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AB322398

Anti-SEMA3B antibody [EPR28920-205]

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Rabbit Recombinant Monoclonal SEMA3B antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

SEMA5, SEMAA, SEMA3B, Semaphorin-3B, Sema A(V), Semaphorin-V, Sema V

4 Images
Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)
  • WB

Supplier Data

Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

A band is seen at ~60 kDa, representing the secreted form of the protein and also band at ~83 kDa, representing the pro-form of the protein.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocelullar carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting SEMA3B whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa,80 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)
  • WB

Supplier Data

Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

SEMA3B High expression : HepG2, SW480.

SEMA3B Low expression : T-47D, PANC-1.

SEMA3C High expression : T-47D, PANC-1.

SEMA3C Low expression : HepG2, SW480.

This antibody is specific to SEMA3B and does not cross react with endogenous SEMA3C

A band is seen at ~60 kDa, representing the secreted form of the protein and also band at ~83 kDa, representing the pro-form of the protein.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398) at 1/1000 dilution

Lane 1:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 30 µg

Lane 4:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa,83 kDa,36 kDa

false

Exposure time: 158s

Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)
  • WB

Supplier Data

Western blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

A band is seen at ~60 kDa, representing the secreted form of the protein and also band at ~83 kDa, representing the pro-form of the protein.

Lanes 1-3 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lane 4 is incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 158 seconds; Lanes 4 : 92 seconds

All lanes:

Western blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 4:

Human placenta tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 4:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa,83 kDa,36 kDa

false

Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)
  • Dot

Supplier Data

Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)

Dot blot analysis of SEMA3B using ab322398 at 1 : 1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : No tagged human SEMA3B fragment
Lane2 : No tagged human SEMA3C fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398) at 1/1000 dilution

Lane 1:

No tagged human SEMA3B fragment

Lane 2:

No tagged human SEMA3C fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

  • Carrier free

    Anti-SEMA3B antibody [EPR28920-205] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28920-205

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has weak cross reaction with human SEMA3C by dot blot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
See full target information SEMA3B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com