Rabbit Recombinant Monoclonal SEMA3B antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.
Images
![Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398), expandable thumbnail](https://content.abcam.com/products/images/sema3b-antibody-epr28920-205-ab322398--dot-blot-img439223.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (AB322398)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Alternative names
SEMA5, SEMAA, SEMA3B, Semaphorin-3B, Sema A(V), Semaphorin-V, Sema V
Rabbit Recombinant Monoclonal SEMA3B antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28920-205
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has weak cross reaction with human SEMA3C by dot blot.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398), expandable thumbnail](https://content.abcam.com/products/images/sema3b-antibody-epr28920-205-ab322398--dot-blot-img439223.jpg "Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398)")
Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398)
Dot blot analysis of SEMA3B using ab322398 at 1:1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: No tagged human SEMA3B fragment
Lane2: No tagged human SEMA3C fragmentExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-SEMA3B antibody [EPR28920-205] (ab322398) at 1/1000 dilution
Lane 1: No tagged human SEMA3B fragment
Lane 2: No tagged human SEMA3C fragment
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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