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AB250177

Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SEMA3D antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ760/PRO1491, SEMA3D, Semaphorin-3D

2 Images
Flow Cytometry (Intracellular) - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (AB250177)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (AB250177)

This data was developed using ab180174, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling SEMA3D using ab180174 at 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (AB250177)
  • WB

Supplier Data

Western blot - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (AB250177)

This data was developed using ab180174, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (<a href='/en-us/products/primary-antibodies/sema3d-antibody-epr13458b-c-terminal-ab180174'>ab180174</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Fetal brain lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

Fetal heart lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 90 kDa

true

  • Unconjugated

    Anti-SEMA3D antibody [EPR13458(B)] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13458(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250177 is the carrier-free version of ab180174.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA3D also known as Semaphorin 3D is a member of the semaphorin protein family. It has a mass of approximately 87 kDa. SEMA3D acts as a signaling protein that plays a role in guiding neuronal and non-neuronal cell processes. It functions by binding to neuropilin and plexin receptors to regulate cell movement and positioning. Expression of SEMA3D occurs in various tissues with significant presence in the nervous system lungs and kidneys.
Biological function summary

SEMA3D influences axonal guidance angiogenesis and immune response. It participates as part of a complex with its receptors regulating signaling pathways that are important for nervous system development and vascular patterning. By altering cellular processes SEMA3D can impact how cells grow connect and interact within their environment affecting tissue morphogenesis and repair.

Pathways

SEMA3D interacts with the semaphorin signaling pathway and the VEGF (vascular endothelial growth factor) pathway. These pathways are essential for processes such as axon guidance and blood vessel formation. SEMA3D works closely with proteins like Plexin-A1 and Neuropilin-1 orchestrating cellular responses necessary for vascular and neural network development.

SEMA3D has connections to cancer progression and pulmonary diseases. Altered expression of SEMA3D can influence tumor growth and metastasis with changes in related proteins such as Neuropilin-1 affecting these processes. In pulmonary disorders SEMA3D expression levels can impact lung development and function linking to conditions like pulmonary fibrosis where the dysregulation of this protein and its receptor Plexin-A1 might play a role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).
See full target information SEMA3D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com