Anti-SEMA4C/SEMAI antibody

• WB

Unknown

Western blot - Anti-SEMA4C/SEMAI antibody (AB135856)

All lanes:

Western blot - Anti-SEMA4C/SEMAI antibody (ab135856) at 1/500 dilution

All lanes:

SW13 cell extract at 30 µg

Predicted band size: 93 kDa

false

• WB

Lab

Western blot - Anti-SEMA4C/SEMAI antibody (AB135856)

Western blot : Anti-SEMA4C antibody (ab135856) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab135856 was shown to bind specifically to SEMA4C. A band was observed at 93 kDa in wild-type A549 cell lysates with no signal observed at this size in SEMA4C knockout cell line. To generate this image, wild-type and SEMA4C knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SEMA4C/SEMAI antibody (ab135856) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

SEMA4C knockout A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 93 kDa

false