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AB281142

Anti-SEMA4C/SEMAI antibody [EPR24010-69] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal SEMA4C/SEMAI antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

KIAA1739, SEMAI, UNQ5855/PRO34487, SEMA4C, Semaphorin-4C

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Sandwich ELISA - Anti-SEMA4C/SEMAI antibody [EPR24010-69] - BSA and Azide free (Capture) (AB281142)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SEMA4C/SEMAI antibody [EPR24010-69] - BSA and Azide free (Capture) (AB281142)

Example of Human SEMA4C standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24010-69

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281142 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SEMA4C. The recommended pair for sandwich ELISA is:
Capture: ab281142, Human SEMA4C Capture Antibody (unconjugated)
Detector: ab280992, Human SEMA4C Detector Antibody (unconjugated)
The reference range value is 0.08 - 10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA4C also known as SEMAI is a member of the semaphorin family which are proteins involved in cellular signaling. SEMA4C has a molecular mass of approximately 89 kDa and features in numerous cellular contexts. It is expressed in various tissues including the neural tissue muscle tissue and certain epithelial tissues. This expression suggests a diverse role in different cellular environments contributing to various physiological processes.
Biological function summary

SEMA4C plays a significant role in mediating cell-cell communication particularly in processes like axon guidance and cell migration. It acts as a ligand in binding to plexin receptors forming part of signaling complexes that regulate cellular dynamics. This protein can influence cytoskeletal reorganization which is also essential for connections between neurons and the development of the nervous system. SEMA4C is important for both developmental processes and responses in adults where its regulation impacts cellular behavior and function.

Pathways

SEMA4C modulates signaling cascades such as the MAPK pathway and the PI3K/AKT pathway. These pathways are critical in controlling growth and survival of cells. SEMA4C interacts with other proteins such as Plexin-B1 and Plexin-B2 signaling through these pathways and impacting neuronal development and immune response. It acts as a mediator between the extracellular environment and intracellular signaling mechanisms playing an important role in cellular responses to external stimuli.

SEMA4C is associated with cancer and neurodegenerative diseases. In cancer overexpression of SEMA4C can promote tumor progression and metastasis often linked with other proteins like Plexin-B1 in the tumor microenvironment. In the context of neurodegenerative diseases alterations or dysregulation of SEMA4C signaling can affect neuronal survival and connectivity and it might interact with proteins like tau contributing to disease progression. Understanding the function of SEMA4C in these conditions may provide avenues for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
See full target information SEMA4C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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