AB312843

Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SEMA4C/SEMAI antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA1739, SEMAI, UNQ5855/PRO34487, SEMA4C, Semaphorin-4C

2 Images

Lab

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free (AB312843)

This data was developed using the same antibody clone in a different buffer formulation (ab312842).

Western blot : Anti-SEMA4C antibody [EPR27116-147] (ab312842) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab312842 was shown to bind specifically to SEMA4C. A band was observed at 93 kDa in wild-type A549 cell lysates with no signal observed at this size in SEMA4C knockout cell line. To generate this image, wild-type and SEMA4C knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] (<a href='/en-us/products/primary-antibodies/sema4c-semai-antibody-epr27116-147-ab312842'>ab312842</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

SEMA4C knockout A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Ramos cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 93 kDa

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Supplier Data

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free (AB312843)

This data was developed using ab312842, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : MG-63 (PMID : 31582836), liver(PMID : 11134026). The molecular weight observed is consistent with what has been described in the literature (PMID : 29555978). The identity of the lower MW band at approximately 18 kDa (in lane 3 and 4) is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution Exposure time : 147 seconds

All lanes:

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] (<a href='/en-us/products/primary-antibodies/sema4c-semai-antibody-epr27116-147-ab312842'>ab312842</a>) at 1/1000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 50 µg

Lane 3:

Human cerebellum tissue lysate at 50 µg

Lane 4:

Human liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 85 kDa,100 kDa

false

Exposure time: 147s

Unconjugated

Anti-SEMA4C/SEMAI antibody [EPR27116-147]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27116-147

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA4C also known as SEMAI is a member of the semaphorin family which are proteins involved in cellular signaling. SEMA4C has a molecular mass of approximately 89 kDa and features in numerous cellular contexts. It is expressed in various tissues including the neural tissue muscle tissue and certain epithelial tissues. This expression suggests a diverse role in different cellular environments contributing to various physiological processes.

Biological function summary

SEMA4C plays a significant role in mediating cell-cell communication particularly in processes like axon guidance and cell migration. It acts as a ligand in binding to plexin receptors forming part of signaling complexes that regulate cellular dynamics. This protein can influence cytoskeletal reorganization which is also essential for connections between neurons and the development of the nervous system. SEMA4C is important for both developmental processes and responses in adults where its regulation impacts cellular behavior and function.

Pathways

SEMA4C modulates signaling cascades such as the MAPK pathway and the PI3K/AKT pathway. These pathways are critical in controlling growth and survival of cells. SEMA4C interacts with other proteins such as Plexin-B1 and Plexin-B2 signaling through these pathways and impacting neuronal development and immune response. It acts as a mediator between the extracellular environment and intracellular signaling mechanisms playing an important role in cellular responses to external stimuli.

SEMA4C is associated with cancer and neurodegenerative diseases. In cancer overexpression of SEMA4C can promote tumor progression and metastasis often linked with other proteins like Plexin-B1 in the tumor microenvironment. In the context of neurodegenerative diseases alterations or dysregulation of SEMA4C signaling can affect neuronal survival and connectivity and it might interact with proteins like tau contributing to disease progression. Understanding the function of SEMA4C in these conditions may provide avenues for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

See full target information SEMA4C

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free (AB312843)

Western blot - Anti-SEMA4C/SEMAI antibody [EPR27116-147] - BSA and Azide free (AB312843)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Proteins & Peptides

Product promise