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AB221620

Anti-SEMA4F antibody

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(1 Publication)

Rabbit Polyclonal SEMA4F antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEMA4F aa 1-150.

View Alternative Names

SEMAM, SEMAW, SEMA4F, Semaphorin-4F, Semaphorin-M, Semaphorin-W, Sema M, Sema W

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SEMA4F antibody (AB221620)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SEMA4F antibody (AB221620)

U-251 MG cells stained for SEMA4F (green) using ab221620 (4 μg/ml) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SEMA4F aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O95754

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA4F also known as Semaphorin-4F is a member of the semaphorin family of proteins. It has an approximate molecular mass of 85 kDa. This protein is expressed mainly in the central nervous system particularly in neurons and glial cells. SEMA4F is a membrane-bound protein that plays a role in axonal guidance interacting with specific cell surface receptors during neural development.
Biological function summary

SEMA4F serves as a mediator in cell signaling processes critical for neuronal connections. It is part of a larger signaling complex that includes plexins and neuropilins which help guide axonal pathfinding and neuronal wiring. SEMA4F influences neurite outgrowth and contributes to the establishment of neural networks by regulating cytoskeletal dynamics and cell adhesion properties.

Pathways

Several cellular signaling mechanisms involve SEMA4F interactions. SEMA4F is involved in the PI3K/Akt and MAPK/ERK pathways both essential for cell growth and survival. Within these pathways SEMA4F interacts with Plexin-B1 which transduces intracellular signals affecting cytoskeleton reorganization. These interactions highlight its role in neurodevelopment and differentiation.

SEMA4F has associations with neurodegenerative diseases and cancer. Aberrant SEMA4F expression has links to Alzheimer's disease where it interacts with proteins like Β-amyloid precursor protein potentially influencing disease progression. In cancer biology abnormal SEMA4F signaling is observed in gliomas with its interactions with Plexin-B1 potentially affecting tumor growth and metastasis. Understanding these connections may provide insights into therapeutic strategies for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable cell surface receptor that regulates oligodendroglial precursor cell migration (By similarity). Might also regulate differentiation of oligodendroglial precursor cells (By similarity). Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).
See full target information SEMA4F
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational andrology and urology 12:176-186 PubMed36915888

2023

Identification and validation of immunohistochemical marker panels to predict the prognosis of muscle invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yidie Ying,Ziwei Wang,Yuxin Tan,Haotian Cao,Hongliang Gao,Zhensheng Zhang,Shuxiong Zeng,Chuanliang Xu
View all publications
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