Rabbit Polyclonal SEMA5A antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEMA5A aa 700-1000.
Images
Publications
Filter by
- Molecular carcinogenesis 61:1177-11902022Tumor-derived extracellular vesicles confer 5-fluorouracil resistance in esophageal cancer via long noncoding RNA AC116025.2 delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuyao Zhang,Shaojie Liu,Jingqing Ren,Hanshuo Zhang,Song Chen,Yun Chen,Shengqi Zhang,Wang Chen,Chengcheng Xu,Shilong Zhong,Sulin Liu,Chaoxian LinPubMed 36239547
- Frontiers in cell and developmental biology 9:5799512021MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGautier Tejedor,Patricia Luz-Crawford,Audrey Barthelaix,Karine Toupet,Sébastien Roudières,François Autelitano,Christian Jorgensen,Farida DjouadPubMed 33738280
- Aging 12:21674-216862020Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wang et. al.PubMed 33176280
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SEMA5A aa 700-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q13591
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Alternative names
SEMAF, SEMA5A, Semaphorin-5A, Semaphorin-F, Sema F
Recommended products
Rabbit Polyclonal SEMA5A antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEMA5A aa 700-1000.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SEMA5A aa 700-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q13591
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEMA5A also known as Semaphorin-5A is a member of the semaphorin family of proteins which guide axonal development and cell migration. This protein has a mass of approximately 89 kDa. SEMA5A is expressed in various tissues including the brain where it plays a significant part in neuronal development. It is a transmembrane protein with specific structural domains that facilitate its role in cell signaling affecting axonal guidance and cellular movement.
- Biological function summary
SEMA5A functions in cell signaling mechanisms essential for proper neural circuit formation and development. The protein interacts with receptors on the cell surface including Plexin-B3 which serves as one of its main receptors and this interaction mediates axonal guidance. Not typically part of a larger complex SEMA5A still influences multiple biological processes such as cell adhesion and migration affecting how neurons connect and communicate in the central nervous system.
- Pathways
SEMA5A operates within important signaling pathways like the semaphorin signaling pathway and the axon guidance pathway. These pathways play significant roles in neural development and involve key interactions with proteins like Plexins and Neuropilins. Within these pathways SEMA5A contributes to the establishment of neural connections and synaptic stability interacting with related proteins that direct cellular growth and structure formation.
- Associated diseases and disorders
Research associates SEMA5A with conditions such as autism spectrum disorder and certain types of cancer. Alterations in SEMA5A expression or function can contribute to neural connectivity irregularities observed in autism. Additionally SEMA5A shows involvement in cancer cell migration and invasion. Its interaction with Plexin-B3 can influence tumor progression and metastasis suggesting potential as a therapeutic target in disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-SEMA5A antibody (ab127002)
5% SDS PAGE
All lanes: Western blot - Anti-SEMA5A antibody (ab127002) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 120 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com