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AB127002

Anti-SEMA5A antibody

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(4 Publications)

Rabbit Polyclonal SEMA5A antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEMA5A aa 700-1000.

View Alternative Names

SEMAF, SEMA5A, Semaphorin-5A, Semaphorin-F, Sema F

1 Images
Western blot - Anti-SEMA5A antibody (AB127002)
  • WB

Unknown

Western blot - Anti-SEMA5A antibody (AB127002)

5% SDS PAGE

All lanes:

Western blot - Anti-SEMA5A antibody (ab127002) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 120 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SEMA5A aa 700-1000. The exact immunogen used to generate this antibody is proprietary information.

Q13591

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA5A also known as Semaphorin-5A is a member of the semaphorin family of proteins which guide axonal development and cell migration. This protein has a mass of approximately 89 kDa. SEMA5A is expressed in various tissues including the brain where it plays a significant part in neuronal development. It is a transmembrane protein with specific structural domains that facilitate its role in cell signaling affecting axonal guidance and cellular movement.
Biological function summary

SEMA5A functions in cell signaling mechanisms essential for proper neural circuit formation and development. The protein interacts with receptors on the cell surface including Plexin-B3 which serves as one of its main receptors and this interaction mediates axonal guidance. Not typically part of a larger complex SEMA5A still influences multiple biological processes such as cell adhesion and migration affecting how neurons connect and communicate in the central nervous system.

Pathways

SEMA5A operates within important signaling pathways like the semaphorin signaling pathway and the axon guidance pathway. These pathways play significant roles in neural development and involve key interactions with proteins like Plexins and Neuropilins. Within these pathways SEMA5A contributes to the establishment of neural connections and synaptic stability interacting with related proteins that direct cellular growth and structure formation.

Research associates SEMA5A with conditions such as autism spectrum disorder and certain types of cancer. Alterations in SEMA5A expression or function can contribute to neural connectivity irregularities observed in autism. Additionally SEMA5A shows involvement in cancer cell migration and invasion. Its interaction with Plexin-B3 can influence tumor progression and metastasis suggesting potential as a therapeutic target in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
See full target information SEMA5A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 61:1177-1190 PubMed36239547

2022

Tumor-derived extracellular vesicles confer 5-fluorouracil resistance in esophageal cancer via long noncoding RNA AC116025.2 delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyao Zhang,Shaojie Liu,Jingqing Ren,Hanshuo Zhang,Song Chen,Yun Chen,Shengqi Zhang,Wang Chen,Chengcheng Xu,Shilong Zhong,Sulin Liu,Chaoxian Lin

Frontiers in cell and developmental biology 9:579951 PubMed33738280

2021

MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Gautier Tejedor,Patricia Luz-Crawford,Audrey Barthelaix,Karine Toupet,Sébastien Roudières,François Autelitano,Christian Jorgensen,Farida Djouad

Aging 12:21674-21686 PubMed33176280

2020

Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Haoran Li,Qingdong Qiao,Yukun Ge,Ling Ma,Qiang Wang

PloS one 11:e0158358 PubMed27355368

2016

HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Kudová,Jiřina Procházková,Ondřej Vašiček,Tomáš Perečko,Miroslava Sedláčková,Martin Pešl,Jiří Pacherník,Lukáš Kubala
View all publications
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Product promise

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