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AB51957

Anti-SEMA5A antibody

3

(2 Reviews)

|

(2 Publications)

Goat Polyclonal SEMA5A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEMA5A aa 50-150.

View Alternative Names

SEMAF, SEMA5A, Semaphorin-5A, Semaphorin-F, Sema F

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (AB51957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA5A antibody (AB51957)

ab51957 at 4μg/ml staining SEMA5A in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was detected by HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SEMA5A aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q13591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab51957 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA5A also known as Semaphorin-5A is a member of the semaphorin family of proteins which guide axonal development and cell migration. This protein has a mass of approximately 89 kDa. SEMA5A is expressed in various tissues including the brain where it plays a significant part in neuronal development. It is a transmembrane protein with specific structural domains that facilitate its role in cell signaling affecting axonal guidance and cellular movement.
Biological function summary

SEMA5A functions in cell signaling mechanisms essential for proper neural circuit formation and development. The protein interacts with receptors on the cell surface including Plexin-B3 which serves as one of its main receptors and this interaction mediates axonal guidance. Not typically part of a larger complex SEMA5A still influences multiple biological processes such as cell adhesion and migration affecting how neurons connect and communicate in the central nervous system.

Pathways

SEMA5A operates within important signaling pathways like the semaphorin signaling pathway and the axon guidance pathway. These pathways play significant roles in neural development and involve key interactions with proteins like Plexins and Neuropilins. Within these pathways SEMA5A contributes to the establishment of neural connections and synaptic stability interacting with related proteins that direct cellular growth and structure formation.

Research associates SEMA5A with conditions such as autism spectrum disorder and certain types of cancer. Alterations in SEMA5A expression or function can contribute to neural connectivity irregularities observed in autism. Additionally SEMA5A shows involvement in cancer cell migration and invasion. Its interaction with Plexin-B3 can influence tumor progression and metastasis suggesting potential as a therapeutic target in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
See full target information SEMA5A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 236:6754-6771 PubMed33788269

2021

HDAC inhibition prevents hypobaric hypoxia-induced spatial memory impairment through PΙ3K/GSK3β/CREB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul Kumar,Vishal Jain,Neetu Kushwah,Aastha Dheer,Kamla Prasad Mishra,Dipti Prasad,Shashi Bala Singh

BMC cancer 12:358 PubMed22901239

2012

Identification of a novel set of genes reflecting different in vivo invasive patterns of human GBM cells.

Applications

ICC/IF

Species

Human

Massimiliano Monticone,Antonio Daga,Simona Candiani,Francesco Romeo,Valentina Mirisola,Silvia Viaggi,Ilaria Melloni,Simona Pedemonte,Gianluigi Zona,Walter Giaretti,Ulrich Pfeffer,Patrizio Castagnola
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Product promise

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