Rabbit Polyclonal Semaphorin 3A antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Rat, Human samples. Cited in 61 publications.
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- Nature metabolism 5:1174-11872023Commensal bacteria weaken the intestinal barrier by suppressing epithelial neuropilin-1 and Hedgehog signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Pontarollo,Bettina Kollar,Amrit Mann,My Phung Khuu,Klytaimnistra Kiouptsi,Franziska Bayer,Inês Brandão,Valeriya V Zinina,Jennifer Hahlbrock,Frano Malinarich,Maximilian Mimmler,Sudhanshu Bhushan,Federico Marini,Wolfram Ruf,Meriem Belheouane,John F Baines,Kristina Endres,Scott M Reba,Verena K Raker,Carsten Deppermann,Christoph Welsch,Markus Bosmann,Natalia Soshnikova,Benoit Chassaing,Mattias Bergentall,Felix Sommer,Fredrik Bäckhed,Christoph ReinhardtPubMed 37414930
- Neoplasma 70:103-1132023Semaphorin 3A inhibits tumor progression via the downregulation of Lin28B in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu-Yue Ma,Li-Na Yang,Ji-Na Chen,Ru-Jun Chen,Li-Wen Zhang,Xiao-Qin WangPubMed 36660796
- Journal of diabetes investigation 14:193-2042022LncRNA TCF7 contributes to high glucose-induced damage in human podocytes by up-regulating SEMA3A via sponging miR-16-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Jiang,Lijie Qian,Ruifeng Yang,Yan Wu,Yongping Guo,Tingfang ChenPubMed 36583231
- Neuron 111:190-201.e82022Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixun Su,Xiaorui Wang,Yujian Yang,Liang Chen,Wenlong Xia,Kimberly K Hoi,Hui Li,Qi Wang,Guangdan Yu,Xiaoying Chen,Shouyu Wang,Yuxin Wang,Lan Xiao,Alexei Verkhratsky,Stephen P J Fancy,Chenju Yi,Jianqin NiuPubMed 36384142
- Experimental and therapeutic medicine 24:6822022Age‑related differences for expression of the nerve‑specific proteins after peripheral nerve injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Obata,Kiyohito Naito,Ayaka Kikui,Shinji Nakamura,Kaori Suzuki,So Kawakita,Takamaru Suzuki,Kenji Goto,Nana Nagura,Yoichi Sugiyama,Isao Nagaoka,Muneaki IshijimaPubMed 36185767
- Oxidative medicine and cellular longevity 2022:47570812022Delivery of miR-654-5p via SonoVue Microbubble Ultrasound Inhibits Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells and Arterial Thrombosis and Stenosis through Targeting TCF21.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang,Xiaoqiang Tang,Yong Zhang,Xiaoqin Wang,Haifeng Shi,Ruohan Yin,Changjie PanPubMed 35910838
- Endocrinology and metabolism (Seoul, Korea) 37:290-3022022Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Jin,Ranran Wang,Shiqiao Peng,Xin Liu,Hanyi Zhang,Xue He,Weiping Teng,Xiaochun TengPubMed 35390249
- Cell death and differentiation 29:1941-19542022Regulation of Semaphorin3A in the process of cutaneous wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zheng,Feng Jiang,Chao Wang,Mengjie Dong,Chundi Wang,Enshi Yan,Yi Wang,Zaiou Zhu,Xianbin Xiong,Xu Ding,Jinhai Ye,Yue He,Hongchuang Zhang,Junbo Zhou,Wei Zhang,Yunong Wu,Xiaomeng SongPubMed 35347234
- eLife 10:2021Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlake A Creighton,Simone Afriyie,Deepa Ajit,Cristine R Casingal,Kayleigh M Voos,Joan Reger,April M Burch,Eric Dyne,Julia Bay,Jeffrey K Huang,E S Anton,Meng-Meng Fu,Damaris N LorenzoPubMed 34812142
- International journal of molecular sciences 22:2021Oligodendrocyte-Specific Deletion of Reduces Cerebellar Inflammation and Neurodegeneration in MOG-Induced EAE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanjithkumar Rajendran,Vinothkumar Rajendran,Mario Giraldo-Velasquez,Fevronia-Foivi Megalofonou,Fynn Gurski,Christine Stadelmann,Srikanth Karnati,Martin BerghoffPubMed 34502405
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Rat | Tested | Expected |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Alternative names
SEMAD, SEMA3A, Semaphorin-3A, Semaphorin III, Sema III
Recommended products
Rabbit Polyclonal Semaphorin 3A antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Rat, Human samples. Cited in 61 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was affinity purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Semaphorin 3A also known as SEMA3A is a secreted protein that plays a role as a guidance cue in the nervous system by binding to receptors like neuropilin-1 and plexin-A. It weighs approximately 95 kDa. Expressed mainly in neurons SEMA3A influences neuronal wiring during development. In addition to neural tissues its expression occurs in endothelial cells and immune cells suggesting a role in vascular and immune system functioning.
- Biological function summary
Semaphorin 3A influences cell migration axonal guidance and organogenesis. It functions as a repulsive guidance molecule that unfavorably influences the direction of growing axons and migrating neurons. SEMA3A does not act alone but is part of a complex involving neuropilin and plexin for effective signal transduction. These signaling complexes ensure that axonal pathways form correctly and aid in correct neural network formation.
- Pathways
Semaphorin 3An importantly integrates into the axon guidance and immune response pathways. In the axon guidance pathway it repulses axons and influences the cytoskeletal dynamics by interacting with proteins such as collapsin response mediator protein (CRMP) and Rac1. SEMA3A participates in immune response through its influence on immune cell migration and positioning ensuring an appropriate immune response.
- Associated diseases and disorders
Anomalies in Semaphorin 3A activity relate to cancer and neuropathic pain. Aberrant SEMA3A expression and signaling can contribute to tumor progression and metastasis often working with neuropilin-1 and vascular endothelial growth factor (VEGF) in cancerous processes. Furthermore changes in SEMA3A signaling pathways have links to neuropathic pain where it collaborates with CRMP-2 to alter pain perception and neuropathic pain pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (ab23393)
ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-Semaphorin 3A antibody (ab23393)
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Lanes 1 - 2: Western blot - Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3 - 4: Anti-Semaphorin 3A antibody (ab25999)
Lanes 1 and 3: Neonatal rat brain
Lanes 2 and 4: Human recombinant Sema3A/Fc chimera (95/125 kDa)
Predicted band size: 89 kDa
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