Anti-Semaphorin 3A antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody (AB23393)

ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• WB

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Western blot - Anti-Semaphorin 3A antibody (AB23393)

Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

Lanes 1 - 2:

Western blot - Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution

Lanes 3 - 4:

Anti-Semaphorin 3A antibody (<a href='/en-us/products/unavailable/semaphorin-3a-antibody-ab25999'>ab25999</a>)

Lanes 1 and 3:

Neonatal rat brain

Lanes 2 and 4:

Human recombinant Sema3A/Fc chimera (95/125 kDa)

Predicted band size: 89 kDa

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