Rabbit Recombinant Monoclonal Semaphorin 3A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Semaphorin 3A antibody [EPR23141-241] - BSA and Azide free (Detector) (AB281134), expandable thumbnail](https://content.abcam.com/products/images/semaphorin-3a-antibody-epr23141-241-bsa-and-azide-free-detector-ab281134--sandwich-elisa-img425699.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Semaphorin 3A antibody [EPR23141-241] - BSA and Azide free (Detector) (AB281134)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Alternative names
Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD
Recommended products
Rabbit Recombinant Monoclonal Semaphorin 3A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23141-241
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab281134 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SEMA3A. The recommended pair for sandwich ELISA is:
Capture: Anti-Semaphorin 3A antibody [EPR23141-209] - BSA and Azide free (Capture) ab281284, Human SEMA3A Capture Antibody (unconjugated)
Detector: ab281134, Human SEMA3A Detector Antibody (unconjugated)
The reference range value is 0.16-10 ng/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Semaphorin 3A also known as SEMA3A is a secreted protein that plays a role as a guidance cue in the nervous system by binding to receptors like neuropilin-1 and plexin-A. It weighs approximately 95 kDa. Expressed mainly in neurons SEMA3A influences neuronal wiring during development. In addition to neural tissues its expression occurs in endothelial cells and immune cells suggesting a role in vascular and immune system functioning.
- Biological function summary
Semaphorin 3A influences cell migration axonal guidance and organogenesis. It functions as a repulsive guidance molecule that unfavorably influences the direction of growing axons and migrating neurons. SEMA3A does not act alone but is part of a complex involving neuropilin and plexin for effective signal transduction. These signaling complexes ensure that axonal pathways form correctly and aid in correct neural network formation.
- Pathways
Semaphorin 3An importantly integrates into the axon guidance and immune response pathways. In the axon guidance pathway it repulses axons and influences the cytoskeletal dynamics by interacting with proteins such as collapsin response mediator protein (CRMP) and Rac1. SEMA3A participates in immune response through its influence on immune cell migration and positioning ensuring an appropriate immune response.
- Associated diseases and disorders
Anomalies in Semaphorin 3A activity relate to cancer and neuropathic pain. Aberrant SEMA3A expression and signaling can contribute to tumor progression and metastasis often working with neuropilin-1 and vascular endothelial growth factor (VEGF) in cancerous processes. Furthermore changes in SEMA3A signaling pathways have links to neuropathic pain where it collaborates with CRMP-2 to alter pain perception and neuropathic pain pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Sandwich ELISA - Anti-Semaphorin 3A antibody [EPR23141-241] - BSA and Azide free (Detector) (ab281134), expandable thumbnail](https://content.abcam.com/products/images/semaphorin-3a-antibody-epr23141-241-bsa-and-azide-free-detector-ab281134--sandwich-elisa-img425699.jpg "Sandwich ELISA - Anti-Semaphorin 3A antibody [EPR23141-241] - BSA and Azide free (Detector) (ab281134)")
Sandwich ELISA - Anti-Semaphorin 3A antibody [EPR23141-241] - BSA and Azide free (Detector) (ab281134)
Sandwich ELISA of Human SEMA3A Antibody Pair - BSA and Azide free ab253724 with the capture antibody Anti-Semaphorin 3A antibody [EPR23141-209] - BSA and Azide free (Capture) ab281284 dilution at 2 µg/mL and detector antibody ab281134 dilution at 0.5 µg/mL.
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