Goat Polyclonal Semaphorin 3E antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEMA3E aa 350-400.
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- Cardiovascular research 113:1763-17752017Semaphorin-3E attenuates neointimal formation via suppressing VSMCs migration and proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie-Hong Wu,Yanan Li,Yi-Fan Zhou,James Haslam,Opoku Nana Elvis,Ling Mao,Yuan-Peng Xia,Bo HuPubMed 29016743
- Arthritis research & therapy 17:2212015Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SEMA3E aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link O15041
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4.00000-6.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog, Pig | Dilution info - | Notes - |
Target data
Function
Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation (By similarity).
Alternative names
KIAA0331, SEMAH, SEMA3E, Semaphorin-3E
Recommended products
Goat Polyclonal Semaphorin 3E antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEMA3E aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SEMA3E aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link O15041
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab112886 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Semaphorin 3E also known as SEMA3E is a secreted protein with a molecular mass of about 90 kDa. It belongs to the semaphorin family which is characterized by a conserved sema domain. SEMA3E is highly expressed in a variety of tissues including the nervous system lungs and heart. Semaphorin 3E interacts with receptors such as Plexin-D1 to send signals that influence cellular processes like migration and adhesion.
- Biological function summary
Semaphorin 3E contributes significantly to cell guidance and development within the nervous system. It plays a role in axon guidance by acting as a chemorepellent directing the growth of neurons to their appropriate targets. SEMA3E also forms functional complexes with neuropilins and plexins to exert its effects on vascular patterning and immune cell function. Furthermore it is involved in regulating angiogenesis influencing endothelial cell movement and blood vessel formation.
- Pathways
The signaling net of Semaphorin 3E includes the axon guidance and angiogenesis pathways. Its relationship with Plexin-D1 is important for transducing repulsive axon guidance cues and modulating endothelial cell function within the vasculature. Additionally interactions with neuropilin-1 and neuropilin-2 enhance the protein's role in vascular development facilitating cross-talk with other signaling cascades like VEGF.
- Associated diseases and disorders
Semaphorin 3E has been linked to cancer progression and cardiovascular diseases. Aberrant expression or function of SEMA3E is often associated with tumor metastasis where it influences cancer cell invasion and dissemination. Its role in cardiovascular diseases is highlighted by its impact on blood vessel formation and remodeling which can contribute to conditions like atherosclerosis. Here its interactions with Plexin-D1 and neuropilins are of great interest as potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3E antibody (ab112886)
ab112886 at 4μg/ml showing membranous staining of Semaphorin 3E in the secretory cells of Paraffin-embedded Human Prostate tissue by Immunohistochemistry. Detection utilised HRP staining.
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