JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277139

Anti-Semaphorin 4D/CD100 antibody [010] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Semaphorin 4D/CD100 antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Sema4d aa 1-750.

View Alternative Names

CD100, Semacl2, Semaj, Sema4d, Semaphorin-4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Sema J

2 Images
Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [010] - BSA and Azide free (AB277139)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [010] - BSA and Azide free (AB277139)

Flow cytometric analysis of Semaphorin 4D/CD100 expression on mouse BABL/c splenocytes. Cells were stained with ab277139 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-Semaphorin 4D/CD100 antibody [010] - BSA and Azide free (AB277139)
  • ICC

Unknown

Immunocytochemistry - Anti-Semaphorin 4D/CD100 antibody [010] - BSA and Azide free (AB277139)

Immunofluorescence staining of Semaphorin 4D/CD100 in mouse spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277139 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

010

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt, ICC

applications

Immunogen

Recombinant Fragment Protein within Mouse Sema4d aa 1-750. The exact immunogen used to generate this antibody is proprietary information.

O09126

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/100", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 4D also known as CD100 is a transmembrane protein that plays important roles in cellular signaling. It belongs to the semaphorin protein family known for their role in axonal guidance. CD100 has a molecular mass of approximately 150 kDa. This protein expresses mainly in the immune system including T cells and B cells but can also be found in certain neurons and endothelial cells. It can exist in both a membrane-bound form and a soluble form which arise from proteolytic cleavage.
Biological function summary

CD100 influences various cellular functions by interacting with multiple receptors such as Plexin-B1 and CD72 as part of a larger signaling complex. It contributes to immune response regulation by promoting T-cell activation and B-cell aggregation which are essential for adaptive immunity. In the nervous system it guides neural development including axon growth and synaptic plasticity. CD100's interaction with Plexin-B1 plays a significant role in these developmental processes often affecting cell morphology and movement.

Pathways

Semaphorin 4D is involved in both immune and neural signaling pathways. In the immune system it influences the PI3K-Akt signaling pathway which impacts cell survival and immune cell activation. It associates with pathways involving Plexin-B1 and RhoA to regulate cytoskeletal dynamics affecting cell motility and growth. Additionally CD100's role in neural pathways intersects with proteins like neuropilin-1 influencing semaphorin-mediated signaling essential for neural connectivity.

Semaphorin 4D has links to cancer and autoimmune diseases. High expression levels of CD100 are observed in several tumor types where it might promote tumor growth and metastasis through its signaling pathways. In autoimmune conditions like multiple sclerosis CD100 affects immune cell interactions and can exacerbate inflammatory responses. Its involvement with proteins such as CD72 and Plexin-B1 in these conditions highlights its contribution to pathogenesis and potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed : 23699507, PubMed : 29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed : 23699507, PubMed : 29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed : 17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity).
See full target information Sema4d
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB282868

Human Semaphorid 4D/CD10 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB199475

Anti-Semaphorin 3A antibody [EPR19367]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody [EPR19367] (AB199475)

  • 4
  • 2
Primary Antibodies

AB169545

Anti-CD105 antibody [EPR10145-12]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (AB169545)

  • 4
  • 3
Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

  • 4
  • 160

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com