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AB275401

Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free

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Rabbit Recombinant Monoclonal Semaphorin 4D/CD100 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples.

View Alternative Names

CD100, C9orf164, SEMAJ, SEMA4D, Semaphorin-4D, A8, BB18, GR3

2 Images
Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (AB275401)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (AB275401)

This data was developed using ab275393, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of PC-3 (human prostate adenocarcinoma epithelial cell, Left) / HDLM-2(Human Hodgkin lymphoma, Right) cells labelling Semaphorin 4D/CD100 with ab275393 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : PC-3 (PMID : 23775445).

Gated on viable cells.

Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (AB275401)
  • WB

Lab

Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] - BSA and Azide free (AB275401)

This data was developed using ab275393, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 23775445).

Negative control : PC-3 (PMID : 23775445)

Exposure time : 136 seconds.

All lanes:

Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (<a href='/en-us/products/primary-antibodies/semaphorin-4d-cd100-antibody-epr23866-69-ab275393'>ab275393</a>) at 1/1000 dilution

Lane 1:

HDLM-2 (Human Hodgkin lymphoma) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 140 kDa

false

  • Unconjugated

    Anti-Semaphorin 4D/CD100 antibody [EPR23866-69]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23866-69

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab275401 is the carrier-free version of ab275393.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 4D also known as CD100 is a transmembrane protein that plays important roles in cellular signaling. It belongs to the semaphorin protein family known for their role in axonal guidance. CD100 has a molecular mass of approximately 150 kDa. This protein expresses mainly in the immune system including T cells and B cells but can also be found in certain neurons and endothelial cells. It can exist in both a membrane-bound form and a soluble form which arise from proteolytic cleavage.
Biological function summary

CD100 influences various cellular functions by interacting with multiple receptors such as Plexin-B1 and CD72 as part of a larger signaling complex. It contributes to immune response regulation by promoting T-cell activation and B-cell aggregation which are essential for adaptive immunity. In the nervous system it guides neural development including axon growth and synaptic plasticity. CD100's interaction with Plexin-B1 plays a significant role in these developmental processes often affecting cell morphology and movement.

Pathways

Semaphorin 4D is involved in both immune and neural signaling pathways. In the immune system it influences the PI3K-Akt signaling pathway which impacts cell survival and immune cell activation. It associates with pathways involving Plexin-B1 and RhoA to regulate cytoskeletal dynamics affecting cell motility and growth. Additionally CD100's role in neural pathways intersects with proteins like neuropilin-1 influencing semaphorin-mediated signaling essential for neural connectivity.

Semaphorin 4D has links to cancer and autoimmune diseases. High expression levels of CD100 are observed in several tumor types where it might promote tumor growth and metastasis through its signaling pathways. In autoimmune conditions like multiple sclerosis CD100 affects immune cell interactions and can exacerbate inflammatory responses. Its involvement with proteins such as CD72 and Plexin-B1 in these conditions highlights its contribution to pathogenesis and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed : 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed : 19788569). Promotes the migration of cerebellar granule cells (PubMed : 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed : 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed : 16055703).
See full target information SEMA4D
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Product promise

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