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Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] is a Rabbit Monoclonal antibody that is used in Semaphorin 4D/CD100 Western Blot. Suitable for Mouse samples.

ab320634 is the only monoclonal antibody for Semaphorin 4D/CD100 with mouse reactivity.

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Images

Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (AB320634), expandable thumbnail
  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (AB320634), expandable thumbnail
  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (AB320634), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Mouse
Tested
Not recommended
Rat
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed:23699507, PubMed:29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed:23699507, PubMed:29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed:17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity).

Alternative names

CD100, Semacl2, Semaj, Sema4d, Semaphorin-4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Sema J

Recommended products

Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] is a Rabbit Monoclonal antibody that is used in Semaphorin 4D/CD100 Western Blot. Suitable for Mouse samples.

ab320634 is the only monoclonal antibody for Semaphorin 4D/CD100 with mouse reactivity.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26247-28
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Semaphorin 4D also known as CD100 is a transmembrane protein that plays important roles in cellular signaling. It belongs to the semaphorin protein family known for their role in axonal guidance. CD100 has a molecular mass of approximately 150 kDa. This protein expresses mainly in the immune system including T cells and B cells but can also be found in certain neurons and endothelial cells. It can exist in both a membrane-bound form and a soluble form which arise from proteolytic cleavage.

Biological function summary

CD100 influences various cellular functions by interacting with multiple receptors such as Plexin-B1 and CD72 as part of a larger signaling complex. It contributes to immune response regulation by promoting T-cell activation and B-cell aggregation which are essential for adaptive immunity. In the nervous system it guides neural development including axon growth and synaptic plasticity. CD100's interaction with Plexin-B1 plays a significant role in these developmental processes often affecting cell morphology and movement.

Pathways

Semaphorin 4D is involved in both immune and neural signaling pathways. In the immune system it influences the PI3K-Akt signaling pathway which impacts cell survival and immune cell activation. It associates with pathways involving Plexin-B1 and RhoA to regulate cytoskeletal dynamics affecting cell motility and growth. Additionally CD100's role in neural pathways intersects with proteins like neuropilin-1 influencing semaphorin-mediated signaling essential for neural connectivity.

Associated diseases and disorders

Semaphorin 4D has links to cancer and autoimmune diseases. High expression levels of CD100 are observed in several tumor types where it might promote tumor growth and metastasis through its signaling pathways. In autoimmune conditions like multiple sclerosis CD100 affects immune cell interactions and can exacerbate inflammatory responses. Its involvement with proteins such as CD72 and Plexin-B1 in these conditions highlights its contribution to pathogenesis and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634), expandable thumbnail

    Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: colon (PMID: 8876214)

    The identity of the lower MW band at approximately 40 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634) at 1/1000 dilution

    Lane 1: Mouse lymph node tissue lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Mouse hippocampus tissue lysate at 20 µg

    Lane 4: Mouse colon tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 120-150 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634), expandable thumbnail

    Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: J774A.1(PMID: 8969198).

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 37170687).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634) at 1/1000 dilution

    Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 120 kDa, 150 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634), expandable thumbnail

    Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 37170687).

    Band around 25kDa was cleavage fragment(PMID: 17244710).

    The identity of the lower MW band at approximately 40 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR26247-28] (ab320634) at 1/1000 dilution

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 culture supernatant at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 25 kDa, 120 kDa, 150 kDa, 36 kDa

    Exposure time: 92s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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