AB23578

Anti-Semaphorin 7a antibody

(2 Reviews)

(24 Publications)

Rabbit Polyclonal Semaphorin 7a antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 24 publications.

View Alternative Names

CD108, Cd108, Semal, Semk1, Sema7a, Semaphorin-7A, Semaphorin-K1, Semaphorin-L, Sema K1, Sema L

2 Images

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Immunoprecipitation - Anti-Semaphorin 7a antibody (AB23578)

Semaphorin 7a was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Semaphorin 7a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23578.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 75kDa : Semaphorin 7a.

All lanes:

Immunoprecipitation - Anti-Semaphorin 7a antibody (ab23578)

Predicted band size: 75 kDa

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Lab

Western blot - Anti-Semaphorin 7a antibody (AB23578)

Gel type : MOPS

Blocking buffer : 2% BSA block

All lanes:

Western blot - Anti-Semaphorin 7a antibody (ab23578) at 1 µg/mL

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse cerebellum tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa,90 kDa

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Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab23578 detects Semaphorin 7a protein at 75 kDa by WB. Semaphorin 7a is a protein that is tethered to the cell membrane via a GPI-anchor and is also glycosylated, therefore it is not surprising that in WB we see some additional bands at ~50 and 100 kDa which are likely to be cleavage fragments and glycosylated protein respectively. Preincubation with the immunizing peptide ab30844 is successful in blocking antibody staining.

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Reactivity data

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Product details

Negative control: HEK cells

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 7a also known as SEMA7A or CD108 is a glycoprotein that has a molecular mass of approximately 80 kDa. This target plays a role in axonal guidance and immune response regulation. It is a member of the semaphorin family which generally acts as signaling molecules. Semaphorin 7a is expressed in various tissues including the immune system nervous system and some epithelial cells. Its expression is especially significant in T-cells and is involved in modulating cell adhesion and migration.

Biological function summary

Semaphorin 7a is implicated in diverse processes such as immune system modulation and nervous system development. It functions as part of a complex involving integrins which mediate cell-to-cell communication and interaction with the extracellular matrix. This protein can bridge the immune and neural signaling pathways indicating its multifunctional nature within organisms. Its interaction with β1 integrins enhances T-cell activation and cytokine production which is important for immune response.

Pathways

Semaphorin 7a integrates into the MAPK and PI3K signaling pathways. These pathways are essential for cell survival migration and apoptosis. In the MAPK pathway Semaphorin 7a impacts downstream signaling through its interaction with ERK proteins. Its role in the PI3K pathway connects it to AKT proteins influencing cell growth and metabolic processes. These connections show its versatility and critical role in signal transduction linked to cellular behaviors.

Semaphorin 7a has been associated with multiple sclerosis and melanoma. In multiple sclerosis altered expression of this protein can affect immune response and neural repair processes. Its connection to melanoma is linked with its role in regulating cell motility and adhesion impacting cancer progression and metastasis. Semaphorin 7a's interactions with proteins like Melanocortin 1 Receptor (MC1R) in melanoma provide insights into therapeutic targets for disease interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

See full target information Sema7a

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1251026 PubMed38094294

2023

Semaphorin 7A is protective during inflammatory peritonitis through integrin receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Körner,David Köhler,Mariella Schneider,Judith M Roth,Tiago F Granja,Claudia Eggstein,Valbona Mirakaj,Peter Rosenberger

Frontiers in cell and developmental biology 11:1261038 PubMed37941899

2023

Dynamic regulation of semaphorin 7A and adhesion receptors in ovarian follicle remodeling and ovulation.

Applications

Unspecified application

Species

Unspecified reactive species

Alaknanda Emery,Kylie R Dunning,Doan T Dinh,Lisa K Akison,Rebecca L Robker,Darryl L Russell

Respiratory research 24:12 PubMed36631871

2023

Impaired cell-cell communication and axon guidance because of pulmonary hypoperfusion during postnatal alveolar development.

Applications

Unspecified application

Species

Unspecified reactive species

Debao Li,Jing Wang,Yuan Fang,Yuqing Hu,Yingying Xiao,Qing Cui,Chuan Jiang,Sijuan Sun,Hao Chen,Lincai Ye,Qi Sun

Neuron 111:190-201.e8 PubMed36384142

2022

Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Xiaorui Wang,Yujian Yang,Liang Chen,Wenlong Xia,Kimberly K Hoi,Hui Li,Qi Wang,Guangdan Yu,Xiaoying Chen,Shouyu Wang,Yuxin Wang,Lan Xiao,Alexei Verkhratsky,Stephen P J Fancy,Chenju Yi,Jianqin Niu

Nature communications 13:6040 PubMed36229455

2022

Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.

Applications

Unspecified application

Species

Unspecified reactive species

Noemie Vilallongue,Julia Schaeffer,Anne-Marie Hesse,Céline Delpech,Béatrice Blot,Antoine Paccard,Elise Plissonnier,Blandine Excoffier,Yohann Couté,Stephane Belin,Homaira Nawabi

Oxidative medicine and cellular longevity 2021:8899274 PubMed34007409

2021

Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunen Liu,Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Hongxu Jin,Mingxiao Hou

Nature communications 11:1315 PubMed32161256

2020

Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb.

Applications

Unspecified application

Species

Unspecified reactive species

David Köhler,Tiago Granja,Julia Volz,Michael Koeppen,Harald F Langer,Georg Hansmann,Ekaterina Legchenko,Tobias Geisler,Tamam Bakchoul,Claudia Eggstein,Helene A Häberle,Bernhard Nieswandt,Peter Rosenberger

Cell biochemistry and function 38:58-65 PubMed31732974

2019

LncRNA LOXL1-AS1/miR-28-5p/SEMA7A axis facilitates pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcun Liu,Cheng Guo,Feng Li,Liping Wu

Frontiers in physiology 9:1718 PubMed30555351

2018

Semaphorin 7A Promotes VEGFA/VEGFR2-Mediated Angiogenesis and Intraplaque Neovascularization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Hu,Yifei Liu,Tao You,Li Zhu

Experimental and therapeutic medicine 15:2356-2364 PubMed29456642

2018

Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Hailing Wang,Kui Jia,Hao Wang,Tao Ren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Semaphorin 7a antibody

Immunoprecipitation - Anti-Semaphorin 7a antibody (AB23578)

Western blot - Anti-Semaphorin 7a antibody (AB23578)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

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