AB139405

Anti-Semenogelin I antibody [EPR3675(2)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Semenogelin I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

SEMG, SEMG1, Semenogelin-1, Cancer/testis antigen 103, Semenogelin I, SGI

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

Immunohistochemical analysis of paraffin-embedded Human seminal vesicle tissue labelling Semenogelin I with ab139405 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

All lanes:

Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (ab139405) at 1/1000 dilution

Lane 1:

Y-79 cell lysate at 10 µg

Lane 2:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

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CiteAb

Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

Semenogelin I western blot using anti-Semenogelin I antibody [EPR3675(2)] ab139405. Publication image and figure legend from Zhou, Q. Z., Guo, X. B., et al., 2019, Andrology, PubMed 30575326.

ab139405 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab139405 please see the product overview.

The mRNA and protein expression levels of SEMG1 were examined by qRT-PCR and Western blots, respectively. (a, b) The SEMG1 mRNA levels were significantly higher in AZS patients compared with that in normozoospermic volunteers (1.40 ± 0.35 vs. 0.46 ± 0.23, p < 0.001). (c, d) The protein expression of SEMG1 in the spermatozoa of AZS patients was significantly higher than that in normozoospermic controls (2.96 ± 0.13 vs. 1.68 ± 0.38, *p < 0.005 and ***p < 0.001).

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Carrier free

Anti-Semenogelin I antibody [EPR3675(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3675(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semenogelin I sometimes abbreviated as SgI acts mechanically as an important component in the formation of the semen coagulum. This protein has a molecular mass of approximately 52 kDa and is expressed chiefly in the seminal vesicles. After ejaculation Semenogelin I contributes significantly to the formation of a gel-like matrix which plays an important role in the initial viscosity of seminal fluid. Upon the cleavage of Semenogelin I by prostate-specific antigen (PSA) this gel liquefies allowing sperm to achieve motility.

Biological function summary

The primary role of Semenogelin I lies in its involvement in semen coagulation and liquefaction processes. It forms a complex with other seminal plasma proteins to stabilize the gel structure. This structural role ensures that Semenogelin I helps regulate the controlled release and proper function of spermatozoa. Additionally it is implicated in modulating immune system responses against male seminal antigens to prevent adverse reactions in the female reproductive tract.

Pathways

The actions of Semenogelin I integrate it into reproductive and coagulation pathways. In the context of semen coagulation and liquefaction it works closely with PSA which breaks down Semenogelin I and facilitates liquefaction and sperm motility. This process ties Semenogelin I into the prostaglandin signaling pathway which affects reproductive physiology and fertility. Furthermore proteins such as fibronectin and lactoferrin relate to Semenogelin I within these pathways assisting in post-ejaculation changes that enhance sperm function and fertility.

Semenogelin I is linked to fertility issues and prostate cancer. Abnormal levels or altered cleavage patterns can contribute to male infertility potentially through improper semen liquefaction that affects sperm motility. In prostate cancer elevated degradation of Semenogelin I by enzymes like PSA could serve as an indicator of tumor activity and progression. Consequently monitoring Semenogelin I alongside PSA levels can offer insights into both male reproductive health and prostate cancer management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.. Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.

See full target information SEMG1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:2112359 PubMed35432561

2022

Baicalin Attenuates Continuous Activation of -Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Fan,Feng Dai,Chunhu Zhang,Lu Zhou,Xinjian Qiu,Yunhui Li

Andrology 8:407-420 PubMed31364287

2019

Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

X Liu,G Liu,P Zhu,Y Wang,J Wang,W Zhang,W Wang,N Li,X Wang,C Zhang,J Liu,X Shen,F Liu

Andrology 7:220-227 PubMed30575326

2018

Expressions of miR-525-3p and its target gene SEMG1 in the spermatozoa of patients with asthenozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Q-Z Zhou,X-B Guo,W-S Zhang,J-H Zhou,C Yang,J Bian,M-K Chen,W-B Guo,P Wang,T Qi,C-Y Wang,J-K Yang,C-D Liu

PLoS genetics 13:e1006743 PubMed28414719

2017

Crucial role of estrogen for the mammalian female in regulating semen coagulation and liquefaction in vivo.

Applications

Species

Mouse

Shuai Li,Marleny Garcia,Rachel L Gewiss,Wipawee Winuthayanon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Semenogelin I antibody [EPR3675(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

Western blot - Anti-Semenogelin I antibody [EPR3675(2)] (AB139405)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise