Anti-SENP1 antibody [EPR3844]

8 product images

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  Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)

  Blocking buffer: 5% NFDM/TBST
  Dilution buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

  All lanes: Daudi cell lysate at 10 µg

  Secondary

  All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

  Predicted band size: 73 kDa

  Observed band size: 73 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981)

  Immunofluorescence staining of Jurkat cells with purified ab108981 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108981 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (ab108981)

  Immunohistochemical staining of paraffin embedded human testis with purified ab108981 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

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  Image courtesy of a customer review submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

  Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (ab108981)

  Unpurified ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

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  Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)

  Blocking buffer: 5% NFDM/TBST
  Dilution buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

  All lanes: U87-MG cell lysate at 10 µg

  Secondary

  All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

  Predicted band size: 73 kDa

  Observed band size: 73 kDa

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  Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)

  All lanes: Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

  Lane 1: HeLa cell lysates at 10 µg

  Lane 2: HUVEC cell lysates at 10 µg

  Lane 3: Jurkat cell lysates at 10 µg

  Lane 4: Daudi cell lysates at 10 µg

  Lane 5: U87-MG cell lysates at 10 µg

  Secondary

  All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

  Predicted band size: 73 kDa

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  This image is courtesy of a customer review submitted by Ragnhild Eskeland

  Western blot - Anti-SENP1 antibody [EPR3844] (ab108981)

  Blocked with 3% milk for 1 hour at 25°C.

  Incubated with the primary antibody for 18 hours at 4°C.

  All lanes: Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

  Lane 1: COS-1 cell lysate at 20000 Cells

  Lane 2: COS-1 cell lysate transfected with SENP1 at 20000 Cells

  Lane 3: COS-1 cell lysate transfected with SENP1 mutant at 20000 Cells

  Secondary

  All lanes: HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 73 kDa

  Observed band size: 75 kDa

  Exposure time: 6min

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  Flow Cytometry (Intracellular) - Anti-SENP1 antibody [EPR3844] (ab108981)

  Intracellular Flow Cytometry analysis of HeLa cells labelling SENP1 with purified ab108981 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.