Rabbit Polyclonal SENP2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SENP2 aa 450-550.
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Publications
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- Nature communications 12:18122021SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXun Shangguan,Jianli He,Zehua Ma,Weiwei Zhang,Yiyi Ji,Kai Shen,Zhiying Yue,Wenyu Li,Zhixiang Xin,Quan Zheng,Ying Cao,Jiahua Pan,Baijun Dong,Jinke Cheng,Qi Wang,Wei XuePubMed 33753739
- PloS one 15:e02426062020Zinc deficiency induces abnormal development of the myocardium by promoting SENP5 overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Zhang et. al.PubMed 33211757
- Molecular brain 13:592020Forebrain excitatory neuron-specific SENP2 knockout mouse displays hyperactivity, impaired learning and memory, and anxiolytic-like behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDehua Huang et. al.PubMed 32290845
- Experimental and therapeutic medicine 19:1855-18632020Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle.
- Frontiers in pharmacology 10:10392019Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yan Zhang et. al.PubMed 31616295
- Biochimica et biophysica acta. Biomembranes 1861:1293-13012019The SUMO-Specific Protease Senp2 Regulates SUMOylation, Expression and Function of Human Organic Anion Transporter 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoxun Wang et. al.PubMed 31054272
- Oncotarget 8:70142-701552017Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonghua Wang et. al.PubMed 29050267
- The Journal of biological chemistry 285:28064-752010Sumoylation regulates interaction of FOG1 with C-terminal-binding protein (CTBP).
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SENP2 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC62
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed:20194620).
Alternative names
Sentrin-specific protease 2, Axam2, SMT3-specific isopeptidase 2, Sentrin/SUMO-specific protease SENP2, Smt3ip2, SENP2, KIAA1331
Recommended products
Rabbit Polyclonal SENP2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SENP2 aa 450-550.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SENP2 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC62
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SENP2 also known as Sentrin/SUMO-specific protease 2 functions as a protease that removes SUMO (Small Ubiquitin-like Modifier) moieties from target proteins. This activity regulates the sumoylation state of proteins affecting their stability activity and localization. SENP2 has a molecular mass of around 63 kDa. It is widely expressed in various tissues including the heart liver and placenta with particularly high levels in these areas suggesting its importance across different biological processes.
- Biological function summary
SUMO E3 ligase complexes often include SENP2 as an integral part. It modulates various signaling pathways by targeting transcription factors and other proteins involved in cellular stress response cell division and apoptosis. Through de-sumoylation SENP2 influences the activity of proteins by altering their transcriptional potency nuclear transport or protein degradation rates. SENP2's activity ensures appropriate cellular responses to environmental cues and maintains proper cellular homeostasis by balancing protein modifications.
- Pathways
SUMO-related mechanisms like nucleocytoplasmic transport and DNA repair involve SENP2. In these pathways SENP2 interacts with proteins like RanBP2 and PML contributing to cell cycle regulation and maintaining genomic stability. In the hypoxia response pathway SENP2's influence is important for modulating hypoxia-inducible factors (HIFs). These interactions illustrate SENP2’s regulatory roles in adapting cellular function in response to stress and changes in the cellular environment.
- Associated diseases and disorders
SENP2 displays relevance in cancer and cardiovascular disorders. Altered sumoylation activity linked with SENP2 is often observed in certain cancers where it may misregulate transcription factors like p53 and c-Myc. In cardiovascular diseases SENP2 interacts with proteins such as NFATc1 impacting cardiac development and function. These associations highlight SENP2's potential as a therapeutic target where modulation of its activity might offer novel treatment avenues for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SENP2 antibody (ab58418)
All lanes: Western blot - Anti-SENP2 antibody (ab58418) at 1/500 dilution
All lanes: MDA-MB-435 cell extract
Predicted band size: 68 kDa
Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody (ab58418)
ICC/IF image of ab58418 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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