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AB96865

Anti-SENP2 antibody

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(1 Publication)

Rabbit Polyclonal SENP2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SENP2 aa 1-50.

View Alternative Names

KIAA1331, SENP2, Sentrin-specific protease 2, Axam2, SMT3-specific isopeptidase 2, Sentrin/SUMO-specific protease SENP2, Smt3ip2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody (AB96865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody (AB96865)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SENP2 antibody (ab96865) at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody (AB96865)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody (AB96865)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SENP2 antibody (ab96865) at 1/200 dilution. Lower image merged with DNA probe.

Western blot - Anti-SENP2 antibody (AB96865)
  • WB

Unknown

Western blot - Anti-SENP2 antibody (AB96865)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SENP2 antibody (ab96865) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

MOLT-4 whole cell lysate at 30 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SENP2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9HC62

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SENP2 also known as Sentrin/SUMO-specific protease 2 functions as a protease that removes SUMO (Small Ubiquitin-like Modifier) moieties from target proteins. This activity regulates the sumoylation state of proteins affecting their stability activity and localization. SENP2 has a molecular mass of around 63 kDa. It is widely expressed in various tissues including the heart liver and placenta with particularly high levels in these areas suggesting its importance across different biological processes.
Biological function summary

SUMO E3 ligase complexes often include SENP2 as an integral part. It modulates various signaling pathways by targeting transcription factors and other proteins involved in cellular stress response cell division and apoptosis. Through de-sumoylation SENP2 influences the activity of proteins by altering their transcriptional potency nuclear transport or protein degradation rates. SENP2's activity ensures appropriate cellular responses to environmental cues and maintains proper cellular homeostasis by balancing protein modifications.

Pathways

SUMO-related mechanisms like nucleocytoplasmic transport and DNA repair involve SENP2. In these pathways SENP2 interacts with proteins like RanBP2 and PML contributing to cell cycle regulation and maintaining genomic stability. In the hypoxia response pathway SENP2's influence is important for modulating hypoxia-inducible factors (HIFs). These interactions illustrate SENP2’s regulatory roles in adapting cellular function in response to stress and changes in the cellular environment.

SENP2 displays relevance in cancer and cardiovascular disorders. Altered sumoylation activity linked with SENP2 is often observed in certain cancers where it may misregulate transcription factors like p53 and c-Myc. In cardiovascular diseases SENP2 interacts with proteins such as NFATc1 impacting cardiac development and function. These associations highlight SENP2's potential as a therapeutic target where modulation of its activity might offer novel treatment avenues for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease that catalyzes two essential functions in the SUMO pathway (PubMed : 11896061, PubMed : 12192048, PubMed : 15296745, PubMed : 20194620, PubMed : 21965678). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed : 15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed : 15296745, PubMed : 20194620, PubMed : 21965678). May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway (By similarity). Deconjugates SUMO2 from MTA1 (PubMed : 21965678). Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed : 20194620). Acts as a regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS and STING1 during the late phase of viral infection (By similarity).
See full target information SENP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 9: PubMed30999631

2019

Regulation of the Expression of DAPK1 by SUMO Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingshui Wang,Xiuli Zhang,Ling Chen,Shuyun Weng,Yun Xia,Yan Ye,Ke Li,Ziqiang Liao,Pengchen Chen,Khaldoon Alsamman,Chen Meng,Craig Stevens,Ted R Hupp,Yao Lin
View all publications
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Product promise

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