AB131637

Anti-SENP2 antibody [OTI1H2]

Name: Anti-SENP2 antibody [OTI1H2] (ab131637) | Abcam
Brand: Abcam Limited
SKU: AB131637
Price: 520 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal SENP2 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SENP2 aa 100-550.

View Alternative Names

KIAA1331, SENP2, Sentrin-specific protease 2, Axam2, SMT3-specific isopeptidase 2, Sentrin/SUMO-specific protease SENP2, Smt3ip2

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human lung carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human prostate carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human prostate tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human pancreas carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human kidney tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling SENP2 with ab131637 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Paraffin-embedded human pancreas tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SENP2 with ab131637 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

Unknown

Western blot - Anti-SENP2 antibody [OTI1H2] (AB131637)

All lanes:

Western blot - Anti-SENP2 antibody [OTI1H2] (ab131637) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY SENP2 cDNA at 5 µg

Predicted band size: 68 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody [OTI1H2] (AB131637)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody [OTI1H2] (AB131637)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SENP2 stained for SENP2 (green) using ab131637 at 1/100 dilution in ICC/IF.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1H2

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human SENP2 aa 100-550. The exact immunogen used to generate this antibody is proprietary information.

Q9HC62

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Clone OTI1H2 (formerly 1H2).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SENP2 also known as Sentrin/SUMO-specific protease 2 functions as a protease that removes SUMO (Small Ubiquitin-like Modifier) moieties from target proteins. This activity regulates the sumoylation state of proteins affecting their stability activity and localization. SENP2 has a molecular mass of around 63 kDa. It is widely expressed in various tissues including the heart liver and placenta with particularly high levels in these areas suggesting its importance across different biological processes.

Biological function summary

SUMO E3 ligase complexes often include SENP2 as an integral part. It modulates various signaling pathways by targeting transcription factors and other proteins involved in cellular stress response cell division and apoptosis. Through de-sumoylation SENP2 influences the activity of proteins by altering their transcriptional potency nuclear transport or protein degradation rates. SENP2's activity ensures appropriate cellular responses to environmental cues and maintains proper cellular homeostasis by balancing protein modifications.

Pathways

SUMO-related mechanisms like nucleocytoplasmic transport and DNA repair involve SENP2. In these pathways SENP2 interacts with proteins like RanBP2 and PML contributing to cell cycle regulation and maintaining genomic stability. In the hypoxia response pathway SENP2's influence is important for modulating hypoxia-inducible factors (HIFs). These interactions illustrate SENP2’s regulatory roles in adapting cellular function in response to stress and changes in the cellular environment.

SENP2 displays relevance in cancer and cardiovascular disorders. Altered sumoylation activity linked with SENP2 is often observed in certain cancers where it may misregulate transcription factors like p53 and c-Myc. In cardiovascular diseases SENP2 interacts with proteins such as NFATc1 impacting cardiac development and function. These associations highlight SENP2's potential as a therapeutic target where modulation of its activity might offer novel treatment avenues for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protease that catalyzes two essential functions in the SUMO pathway (PubMed : 11896061, PubMed : 12192048, PubMed : 15296745, PubMed : 20194620, PubMed : 21965678). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed : 15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed : 15296745, PubMed : 20194620, PubMed : 21965678). May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway (By similarity). Deconjugates SUMO2 from MTA1 (PubMed : 21965678). Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed : 20194620). Acts as a regulator of the cGAS-STING pathway by catalyzing desumoylation of CGAS and STING1 during the late phase of viral infection (By similarity).

See full target information SENP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39937027

2025

Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Fergan Imbert,Dianne Langford

PloS one 15:e0242606 PubMed33211757

2020

Zinc deficiency induces abnormal development of the myocardium by promoting SENP5 overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhang,Cuancuan Wang,Dan Zhao,Xuhong Chen,Chunyan Zhang,Jun Zheng,Xiaozhi Liu

PloS one 14:e0217578 PubMed31125383

2019

Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency.

Applications

Unspecified application

Species

Unspecified reactive species

Gretchen L Bentz,Angela J Lowrey,Dustin C Horne,Vy Nguyen,Austin R Satterfield,Tabithia D Ross,Abigail E Harrod,Olga N Uchakina,Robert J McKallip

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SENP2 antibody [OTI1H2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (AB131637)

Western blot - Anti-SENP2 antibody [OTI1H2] (AB131637)

Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody [OTI1H2] (AB131637)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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