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AB124790

Anti-SENP3 antibody [EP4360]

5

(2 Reviews)

|

(5 Publications)

Rabbit Monoclonal SENP3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

SSP3, SUSP3, SENP3, Sentrin-specific protease 3, SUMO-1-specific protease 3, Sentrin/SUMO-specific protease SENP3

1 Images
Western blot - Anti-SENP3 antibody [EP4360] (AB124790)
  • WB

Unknown

Western blot - Anti-SENP3 antibody [EP4360] (AB124790)

All lanes:

Western blot - Anti-SENP3 antibody [EP4360] (ab124790) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 65 kDa

false

  • Carrier free

    Anti-SENP3 antibody [EP4360] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4360

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SENP3 also known as SUMO-specific protease 3 is a protein with a molecular mass of about 66 kDa. It functions primarily as a protease that reverses the SUMOylation of target proteins by cleaving SUMO residues which modulate protein functions and interactions. SENP3 localizes mostly in the nucleoplasm but can also be found in the cytoplasm. The expression of SENP3 varies across different tissues displaying higher levels in the liver heart and skeletal muscle indicating its role in these tissues’ functions.
Biological function summary

SENP3 modulates SUMOylated proteins' activity influencing cellular processes such as transcription signal transduction and stress response. It is part of a larger complex with nucleophosmin a nucleolar protein that assists SENP3 in stability and substrate localization. SENP3's regulation of SUMOylation is critical for maintaining cellular homeostasis and influencing the outcome of signaling pathways related to various stimuli.

Pathways

The activity of SENP3 affects the SUMOylation cycle an important cellular mechanism for post-translational modification. SENP3 plays a role in the DNA damage response pathway where it associates with proteins like NEMO modifying their activity to facilitate repair processes. Additionally SENP3 participates in the oxidative stress response pathway where its action on transcription factors helps cells adapt to stress conditions effectively.

SENP3 is linked to cancer and cardiovascular disease. In certain cancers including hepatocellular carcinoma SENP3 levels are often upregulated leading to altered transcriptional activity and promoting tumor growth. SENP3 also interacts with proteins such as MDM2 influencing p53 degradation and impacting cell cycle regulation. In cardiovascular disease SENP3 contributes to cardiac hypertrophy through its effects on hypertrophic signaling pathways making it a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates (PubMed : 16608850, PubMed : 32832608, PubMed : 36050397). Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability (PubMed : 15743823). Deconjugates SUMO2 and SUMO3 from CDCA8 (PubMed : 18946085). Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300 (PubMed : 19680224). Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 (PubMed : 19015314). Plays a role in the regulation of sumoylation status of ZNF148 (PubMed : 18259216). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed : 22872859). Deconjugates SUMO2 from KAT5 (PubMed : 32832608). Catalyzes desumoylation of MRE11 (PubMed : 36050397).
See full target information SENP3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genomics, proteomics & bioinformatics 21:1246-1259 PubMed37121272

2023

The Proteome Landscape of Human Placentas for Monochorionic Twins with Selective Intrauterine Growth Restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Lu Meng,Peng-Bo Yuan,Xue-Ju Wang,Jing Hang,Xiao-Ming Shi,Yang-Yu Zhao,Yuan Wei

Kidney diseases (Basel, Switzerland) 8:424-435 PubMed36466072

2022

SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Lirui Wang,Jiangtao Li,Chen Yu

MedComm 3:e123 PubMed35356800

2022

SENP3-mediated TIP60 deSUMOylation is required for DNA-PKcs activity and DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Han,Xin Huang,Xiaoyu Cao,Yuchen Li,Lei Gao,Jin Jia,Gang Li,Hejiang Guo,Xiaochang Liu,Hongling Zhao,Hua Guan,Pingkun Zhou,Shanshan Gao

Oncology letters 21:274 PubMed33732350

2021

Inhibition of SUMO2/3 antagonizes isoflurane-induced cancer-promoting effect in hepatocellular carcinoma Hep3B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Na Xue,Chunyan Zhang,Shimin Shan,Zhongmin Jiang,Wenhan Wu,Xiaozhi Liu

Science advances 6:eaba7822 PubMed32832608

2020

TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Shan Gao,Hua Guan,Shuang Yan,Sai Hu,Man Song,Zong-Pei Guo,Da-Fei Xie,Yike Liu,Xiaodan Liu,Shimeng Zhang,Ping-Kun Zhou
View all publications
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Product promise

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