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AB124840

Anti-SEP15 antibody [NCIR128A]

3

(1 Review)

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(10 Publications)

Rabbit Recombinant Monoclonal SEP15 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

View Alternative Names

SEP15, SELENOF, Selenoprotein F, 15 kDa selenoprotein

4 Images
Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)
  • WB

Supplier Data

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution

All lanes:

Human testis lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)
  • WB

Supplier Data

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression or negative samples : skeletal muscle, MCF7, T-47D (PMID : 10945981, PMID : 35082382).

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)
  • WB

Unknown

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution

Lane 1:

Rat liver lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

LnCaP lysate at 10 µg

Predicted band size: 18 kDa

false

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)
  • WB

Unknown

Western blot - Anti-SEP15 antibody [NCIR128A] (AB124840)

Blocking/Dilution buffer : 5% NFDM/TBST

C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution

Lane 1:

293T transfected with C-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate at 15 µg

Lane 3:

293T transfected with N-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg

Lane 4:

Human SEP15 full length recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-SEP15 antibody [NCIR128A] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

NCIR128A

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

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Primary Antibodies

AB124879

Anti-UGGT/UGT1 antibody [EPR5105]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

  • 0
  • 0
Primary Antibodies

AB26320

Anti-Thioredoxin / TRX antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (AB26320)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.</p>" } } }
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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SEP15 protein also known as Selenoprotein F has a molecular mass of approximately 15 kDa. It plays a mechanical role as a thiol-disulfide oxidoreductase enzyme participating in the formation and isomerization of disulfide bonds within proteins. SEP15 is found mainly in the endoplasmic reticulum (ER) and shows a higher expression level in liver prostate kidney and colon tissues. Its function is strongly linked to the presence of the rare amino acid selenocysteine which is essential for its activity.
Biological function summary

SEP15 influences protein folding and maintenance of redox homeostasis within the endoplasmic reticulum. As part of the UDP-glucose:glycoprotein glucosyltransferase complex it ensures proper protein folding and glycosylation. This role underlies its involvement in quality control of proteins preventing accumulation of misfolded proteins that could be harmful to cells. Its presence is significant in stress response mechanisms helping cells manage oxidative stress conditions.

Pathways

Several studies connect SEP15 to the ER stress signaling pathways and the antioxidant response. In the ER stress response it works alongside proteins like ERO1 and PDI helping ensure proper protein disulfide bond formation. Within antioxidant pathways SEP15 acts in coordination with Se-dependent glutathione peroxidases which protect cells against oxidative damage by reducing peroxides.

SEP15 relates to cancer and neurological disorders. Its malfunction or altered expression can affect tumor development and progression especially in colon cancer where it is often downregulated. Additionally because SEP15 contributes to neuron protection under oxidative stress dysfunction in SEP15 may link to neurodegenerative diseases. This relation further connects to other selenoproteins like GPX1 and GPX4 which play protective roles against oxidative damage in cellular environments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in redox reactions associated with the formation of disulfide bonds (By similarity). May contribute to the quality control of protein folding in the endoplasmic reticulum (PubMed : 24415556). May regulate protein folding by enhancing the catalytic activity of UGGT1/UGCGL1 and UGGT2/UGCGL2 (PubMed : 24415556).
See full target information SELENOF
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Biological trace element research : PubMed40555955

2025

Supranutritional Selenomethionine but not Selenite Reduces Malignant Cell Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Meyer,Maria Schwarz,Felix B Meyer,René Thierbach,Anna P Kipp

Cancers 15: PubMed37509331

2023

SELENOF Controls Proliferation and Cell Death in Breast-Derived Immortalized and Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Roudy C Ekyalongo,Brenna Flowers,Tanu Sharma,Alexandra Zigrossi,An Zhang,Anaisa Quintanilla-Arteaga,Kanishka Singh,Irida Kastrati

The Journal of biological chemistry 298:102677 PubMed36336075

2022

A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yonatan G Mideksa,Isabel Aschenbrenner,Anja Fux,Dinah Kaylani,Caroline A M Weiß,Tuan-Anh Nguyen,Nina C Bach,Kathrin Lang,Stephan A Sieber,Matthias J Feige

International journal of molecular sciences 22: PubMed34298926

2021

Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan Sonet,Anne-Laure Bulteau,Zahia Touat-Hamici,Maurine Mosca,Katarzyna Bierla,Sandra Mounicou,Ryszard Lobinski,Laurent Chavatte

Journal of biological inorganic chemistry : JBIC : 25:1009-1022 PubMed32995962

2020

Selenoprotein F knockout leads to glucose and lipid metabolism disorders in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiang Zheng,Bingyu Ren,Xiaoming Li,Huanhuan Yan,Qingguo Xie,Hongmei Liu,Jun Zhou,Jing Tian,Kaixun Huang

Cell reports 23:1387-1398 PubMed29719252

2018

Role of Selenof as a Gatekeeper of Secreted Disulfide-Rich Glycoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Hee Yim,Robert A Everley,Frank A Schildberg,Sang-Goo Lee,Andrea Orsi,Zachary R Barbati,Kutay Karatepe,Dmitry E Fomenko,Petra A Tsuji,Hongbo R Luo,Steven P Gygi,Roberto Sitia,Arlene H Sharpe,Dolph L Hatfield,Vadim N Gladyshev

Biochimica et biophysica acta. General subjects 1862:2493-2505 PubMed29660373

2018

Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.

Applications

Unspecified application

Species

Human

Zahia Touat-Hamici,Anne-Laure Bulteau,Juliusz Bianga,Hélène Jean-Jacques,Joanna Szpunar,Ryszard Lobinski,Laurent Chavatte

The Prostate 78:279-288 PubMed29314169

2018

Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dede N Ekoue,Emmanuel Ansong,Li Liu,Virgilia Macias,Ryan Deaton,Craig Lacher,Matthew Picklo,Larisa Nonn,Peter H Gann,Andre Kajdacsy-Balla,Gail S Prins,Vincent L Freeman,Alan M Diamond

PloS one 10:e0124487 PubMed25886253

2015

The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways.

Applications

Unspecified application

Species

Human

Petra A Tsuji,Bradley A Carlson,Min-Hyuk Yoo,Salvador Naranjo-Suarez,Xue-Ming Xu,Yiwen He,Esther Asaki,Harold E Seifried,William C Reinhold,Cindy D Davis,Vadim N Gladyshev,Dolph L Hatfield

The Journal of biological chemistry 288:19401-13 PubMed23696641

2013

Translational redefinition of UGA codons is regulated by selenium availability.

Applications

WB

Species

Mouse

Michael T Howard,Bradley A Carlson,Christine B Anderson,Dolph L Hatfield
View all publications
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Product promise

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