Anti-SEP15 antibody [NCIR128A] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SEP15 antibody [NCIR128A] - BSA and Azide free (AB232465)

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124840).

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (<a href='/en-us/products/primary-antibodies/sep15-antibody-ncir128a-ab124840'>ab124840</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-SEP15 antibody [NCIR128A] - BSA and Azide free (AB232465)

This data was developed using ab124840, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression or negative samples : skeletal muscle, MCF7, T-47D (PMID : 10945981, PMID : 35082382).

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (<a href='/en-us/products/primary-antibodies/sep15-antibody-ncir128a-ab124840'>ab124840</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-SEP15 antibody [NCIR128A] - BSA and Azide free (AB232465)

This data was developed using ab124840, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SEP15 antibody [NCIR128A] (<a href='/en-us/products/primary-antibodies/sep15-antibody-ncir128a-ab124840'>ab124840</a>) at 1/1000 dilution

Lane 1:

293T transfected with C-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate at 15 µg

Lane 3:

293T transfected with N-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg

Lane 4:

Human SEP15 full length recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Exposure time: 3s