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AB52158

Anti-Separase antibody

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(1 Publication)

Rabbit Polyclonal Separase antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ESPL1.

View Alternative Names

ESP1, KIAA0165, ESPL1, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Separase antibody (AB52158)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Separase antibody (AB52158)

Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1 : 50 dilution). Left : sample untreated. Right : sample treated with immunizing peptide (negative control).

Western blot - Anti-Separase antibody (AB52158)
  • WB

Unknown

Western blot - Anti-Separase antibody (AB52158)

All lanes:

Western blot - Anti-Separase antibody (ab52158) at 1/500 dilution

Lane 1:

extracts from A431 cells

Lane 2:

extracts from A431 cells, with immunizing peptide

Predicted band size: 233 kDa

Observed band size: 233 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ESPL1. The exact immunogen used to generate this antibody is proprietary information.

Q14674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Separase also known as ESPL1 is a cysteine protease weighing around 230 kDa. It plays an important mechanical role in the cell cycle specifically during the anaphase of mitosis. Separase executes its function by cleaving the cohesin complex which is important for sister chromatid separation. This protein is expressed in a variety of tissues predominantly during cell division. Separase's activity is tightly regulated by securin and cyclin-dependent kinases to prevent premature chromatid separation.
Biological function summary

Separase contributes to the essential process of chromosome segregation by acting as a part of the chromosomal passenger complex. This complex also includes aurora B kinase and survivin which coordinate chromosomal and cytoskeletal dynamics during mitosis. Separase’s activation enables proper anaphase progression and genomic stability as it ensures timely dissolution of cohesin bonds holding sister chromatids together. Its regulation prevents errors in chromosomal distribution that could lead to aneuploidy.

Pathways

Separase integrates into the mitotic spindle checkpoint and DNA damage response pathways critical for maintaining genomic integrity. Aurora B kinase a related protein phosphorylates components of these pathways to coordinate division processes accurately. Separase by ensuring correct chromosomal segregation plays a pivotal role in preventing propagation of cells with damaged DNA or chromosomal abnormalities.

Separase dysfunction links to cancer and congenital cohesinopathies. Abnormal Separase activity can lead to improper chromatid separation contributing to genomic instability a hallmark of cancer. For instance dysregulation of securin a regulatory protein of Separase could lead to tumorigenesis. Additionally defects in separase activity associate with cohesinopathies a group of disorders characterized by developmental anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
See full target information ESPL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Traffic (Copenhagen, Denmark) 12:615-26 PubMed21272169

2011

Securin and separase modulate membrane traffic by affecting endosomal acidification.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Bacac,Carlo Fusco,Anne Planche,Jaime Santodomingo,Nicolas Demaurex,Ruzanna Leemann-Zakaryan,Paolo Provero,Ivan Stamenkovic
View all publications
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Product promise

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