Anti-Separase antibody [XJ11-1B12]
4
(4 Reviews)
|
(17 Publications)
Mouse Monoclonal Separase antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.
View Alternative Names
ESP1, KIAA0165, ESPL1, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase
- WB
Project8302****
Western blot - Anti-Separase antibody [XJ11-1B12] (AB16170)
All lanes:
Western blot - Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/mL
All lanes:
MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
All lanes:
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 233 kDa
Observed band size: 100 kDa,170 kDa,70 kDa
true
Exposure time: 3min
Reactivity data
Product details
Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Separase contributes to the essential process of chromosome segregation by acting as a part of the chromosomal passenger complex. This complex also includes aurora B kinase and survivin which coordinate chromosomal and cytoskeletal dynamics during mitosis. Separase’s activation enables proper anaphase progression and genomic stability as it ensures timely dissolution of cohesin bonds holding sister chromatids together. Its regulation prevents errors in chromosomal distribution that could lead to aneuploidy.
Pathways
Separase integrates into the mitotic spindle checkpoint and DNA damage response pathways critical for maintaining genomic integrity. Aurora B kinase a related protein phosphorylates components of these pathways to coordinate division processes accurately. Separase by ensuring correct chromosomal segregation plays a pivotal role in preventing propagation of cells with damaged DNA or chromosomal abnormalities.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (17)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 25: PubMed38203427
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 14:6088 PubMed37773176
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cell death and differentiation 30:2151-2166 PubMed37596441
2023
Applications
Unspecified application
Species
Unspecified reactive species
Developmental cell 56:2192-2206.e8 PubMed34331869
2021
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 34:108652 PubMed33472072
2021
Applications
Unspecified application
Species
Unspecified reactive species
EMBO reports 19: PubMed29925526
2018
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 8:15803 PubMed28607478
2017
Applications
WB
Species
Human
Nature communications 6:10076 PubMed26647647
2015
Applications
WB
Species
Unspecified reactive species
PloS one 10:e0138905 PubMed26407333
2015
Applications
WB
Species
Unspecified reactive species
Biology open 4:484-95 PubMed25750436
2015
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com