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AB16170

Anti-Separase antibody [XJ11-1B12]

4

(4 Reviews)

|

(17 Publications)

Mouse Monoclonal Separase antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.

View Alternative Names

ESP1, KIAA0165, ESPL1, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase

1 Images
Western blot - Anti-Separase antibody [XJ11-1B12] (AB16170)
  • WB

Project8302****

Western blot - Anti-Separase antibody [XJ11-1B12] (AB16170)

All lanes:

Western blot - Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/mL

All lanes:

MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 233 kDa

Observed band size: 100 kDa,170 kDa,70 kDa

true

Exposure time: 3min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

XJ11-1B12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Used at a dilution of 1/100 for 1 hr on HeLa cells (see Abreview).</p>" } } }

Product details

Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Separase also known as ESPL1 is a cysteine protease weighing around 230 kDa. It plays an important mechanical role in the cell cycle specifically during the anaphase of mitosis. Separase executes its function by cleaving the cohesin complex which is important for sister chromatid separation. This protein is expressed in a variety of tissues predominantly during cell division. Separase's activity is tightly regulated by securin and cyclin-dependent kinases to prevent premature chromatid separation.
Biological function summary

Separase contributes to the essential process of chromosome segregation by acting as a part of the chromosomal passenger complex. This complex also includes aurora B kinase and survivin which coordinate chromosomal and cytoskeletal dynamics during mitosis. Separase’s activation enables proper anaphase progression and genomic stability as it ensures timely dissolution of cohesin bonds holding sister chromatids together. Its regulation prevents errors in chromosomal distribution that could lead to aneuploidy.

Pathways

Separase integrates into the mitotic spindle checkpoint and DNA damage response pathways critical for maintaining genomic integrity. Aurora B kinase a related protein phosphorylates components of these pathways to coordinate division processes accurately. Separase by ensuring correct chromosomal segregation plays a pivotal role in preventing propagation of cells with damaged DNA or chromosomal abnormalities.

Separase dysfunction links to cancer and congenital cohesinopathies. Abnormal Separase activity can lead to improper chromatid separation contributing to genomic instability a hallmark of cancer. For instance dysregulation of securin a regulatory protein of Separase could lead to tumorigenesis. Additionally defects in separase activity associate with cohesinopathies a group of disorders characterized by developmental anomalies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
See full target information ESPL1

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38203427

2024

Hexavalent Chromium Targets Securin to Drive Numerical Chromosome Instability in Human Lung Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer H Toyoda,Julieta Martino,Rachel M Speer,Idoia Meaza,Haiyan Lu,Aggie R Williams,Alicia M Bolt,Joseph Calvin Kouokam,Abou El-Makarim Aboueissa,John Pierce Wise

Nature communications 14:6088 PubMed37773176

2023

Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1.

Applications

Unspecified application

Species

Unspecified reactive species

Devashish Dwivedi,Daniela Harry,Patrick Meraldi

Cell death and differentiation 30:2151-2166 PubMed37596441

2023

Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyu Song,Eun Ho Kim,Jihee Hong,Dasom Gwon,Jee Won Kim,Gyu-Un Bae,Chang-Young Jang

Developmental cell 56:2192-2206.e8 PubMed34331869

2021

Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition.

Applications

Unspecified application

Species

Unspecified reactive species

Nolan K Maier,Jun Ma,Michael A Lampson,Iain M Cheeseman

Cell reports 34:108652 PubMed33472072

2021

Prolonged mitosis causes separase deregulation and chromosome nondisjunction.

Applications

Unspecified application

Species

Unspecified reactive species

Norihisa Shindo,Makoto Otsuki,Kazuhiko S K Uchida,Toru Hirota

EMBO reports 19: PubMed29925526

2018

Adducin-1 is essential for spindle pole integrity through its interaction with TPX2.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hsin Hsu,Won-Jing Wang,Wan-Yi Lin,Yu-Min Huang,Chien-Chen Lai,Jung-Chi Liao,Hong-Chen Chen

Nature communications 8:15803 PubMed28607478

2017

Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay.

Applications

WB

Species

Human

Menuka Karki,Neda Keyhaninejad,Charles B Shuster

Nature communications 6:10076 PubMed26647647

2015

PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit.

Applications

WB

Species

Unspecified reactive species

Jaeyoun Kim,Kwanwoo Lee,Kunsoo Rhee

PloS one 10:e0138905 PubMed26407333

2015

Integrity of the Pericentriolar Material Is Essential for Maintaining Centriole Association during M Phase.

Applications

WB

Species

Unspecified reactive species

Mi Young Seo,Wonyul Jang,Kunsoo Rhee

Biology open 4:484-95 PubMed25750436

2015

MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Voets,Rob Wolthuis
View all publications

Product promise

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