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AB69314

Sepharose Anti-GFP antibody

4

(1 Review)

|

(30 Publications)

Rabbit Polyclonal GFP antibody - conjugated to Sepharose. Suitable for IP.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

1 Images
Immunoprecipitation - Sepharose Anti-GFP antibody (AB69314)
  • IP

Unknown

Immunoprecipitation - Sepharose Anti-GFP antibody (AB69314)

Lane 1 & 3 : IP from COS 7 cells transfected with EGFP.N-Ras (48 kDa)

Lane 2 & 4 : IP from untransfected COS 7 cells

Lane 1 & 2 : IP using 15 ul of rabbit anti-GFP conjugated to sepharose beads (0.5 mg IgG per ml of beads)

Lane 3 & 4 : IP using 15 ul of goat anti-GFP conjugated to sepharose beads (1 mg IgG per ml of beads)

Immunoprecipitation : COS 7 cell lysates containing 100 ug of total protein in 200 ul of 0.1% SDS-RIPA buffer with addition of complete protease inhibitor were used for each immunoprecipitation. Cell lysates were incubated with anti-GFP sepharose beads for 2 hours at 4oC with rocking. Beads were washed. Proteins were eluted with 1% SDS 50 mM HEPES (pH 7.4) at 80oC (15 min). Half of the IP sample was loaded on each lane of 10 % SDS PAGE gel and gel was processed for Western blotting/ECL.

Western blot : Primary antibody : monoclonal mouse anti-GFP (ab291) at 0.2 ug/ml in 5% non-fat milk/TBS-T; 1 hour incubation at room

All lanes:

Immunoprecipitation - Sepharose Anti-GFP antibody (ab69314)

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Sepharose

Excitation/Emission
Carrier free

No

Applications

IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

Specificity

This product is a suspension of affinity purified anti-GFP antibody (ab6556) covalently linked to sepharose beads. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP, and EGFP. The unit sold contains 25μg of affinity purified rabbit anti-GFP IgG cross-linked to 125 μl sepharose beads in a total volume of 250 μl buffer. The product is supplied as a 50% slurry to facilitate pipetting. Pipetting the slurry is facilitated when pipet tips are blunted by cutting 3-5mm off from the tip.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>We rountinely recommend using 20 μL of slurry per IP, which corresponds to 2 μg of IgG cross-linked to beads. this results in a clearly visible 10 μL bead pellet upon centrifugation. Unconjugated version: <a href='/en-us/products/primary-antibodies/gfp-antibody-ab6556'>ab6556</a>. FITC conjugated version: <a href='/en-us/products/unavailable/gfp-antibody-fitc-ab66180'>ab66180</a>. Biotin conjugated version: <a href='/en-us/products/unavailable/gfp-antibody-biotin-ab69313'>ab69313</a>.</p>" } } }
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Product details

This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

The Biochemical journal 481:481-498 PubMed38440860

2024

Evidence that Xrn1 is in complex with Gcn1, and is required for full levels of eIF2α phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Renuka Shanmugam,Reuben Anderson,Anja H Schiemann,Evelyn Sattlegger

Immunity 55:1645-1662.e7 PubMed35882236

2022

Keratinocyte-derived defensins activate neutrophil-specific receptors Mrgpra2a/b to prevent skin dysbiosis and bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xintong Dong,Nathachit Limjunyawong,Elizabeth I Sypek,Gaofeng Wang,Roger V Ortines,Christine Youn,Martin P Alphonse,Dustin Dikeman,Yu Wang,Mark Lay,Ruchita Kothari,Chirag Vasavda,Priyanka Pundir,Loyal Goff,Lloyd S Miller,Wuyuan Lu,Luis A Garza,Brian S Kim,Nathan K Archer,Xinzhong Dong

Diabetes 71:1246-1260 PubMed35377441

2022

DOC2b Enhances β-Cell Function via a Novel Tyrosine Phosphorylation-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Diti Chatterjee Bhowmick,Arianne Aslamy,Supriyo Bhattacharya,Eunjin Oh,Miwon Ahn,Debbie C Thurmond

eLife 10: PubMed34397383

2021

A CTP-dependent gating mechanism enables ParB spreading on DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Sb Jalal,Ngat T Tran,Clare Em Stevenson,Afroze Chimthanawala,Anjana Badrinarayanan,David M Lawson,Tung Bk Le

Nature chemical biology 17:856-864 PubMed33927411

2021

ABHD17 regulation of plasma membrane palmitoylation and N-Ras-dependent cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jarrett R Remsberg,Radu M Suciu,Noemi A Zambetti,Thomas W Hanigan,Ari J Firestone,Anagha Inguva,Amanda Long,Nhi Ngo,Kenneth M Lum,Cassandra L Henry,Stewart K Richardson,Marina Predovic,Ben Huang,Melissa M Dix,Amy R Howell,Micah J Niphakis,Kevin Shannon,Benjamin F Cravatt

Cells 9: PubMed32093146

2020

An Alternative Splice Variant of HIPK2 with Intron Retention Contributes to Cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Gatti,Manuela Ferrara,Ilaria Virdia,Silvia Matteoni,Laura Monteonofrio,Simona di Martino,Maria Grazia Diodoro,Giuliana Di Rocco,Cinzia Rinaldo,Silvia Soddu

Journal of hematology & oncology 12:79 PubMed31324208

2019

Protein arginine methyltransferase 3-induced metabolic reprogramming is a vulnerable target of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Chuan Hsu,Ya-Li Tsai,Chia-Hsien Lin,Mei-Ren Pan,Yan-Shen Shan,Tsung-Yen Cheng,Skye Hung-Chun Cheng,Li-Tzong Chen,Wen-Chun Hung

Molecular biology of the cell 30:1020-1036 PubMed30726152

2019

Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Prashant K Mishra,Gudjon Olafsson,Lars Boeckmann,Timothy J Westlake,Ziad M Jowhar,Lauren E Dittman,Richard E Baker,Damien D'Amours,Peter H Thorpe,Munira A Basrai

Cell death & disease 9:806 PubMed30042418

2018

XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-In Jeong,Jung-Wook Kim,Kyung-Phil Ko,Byung-Kyu Ryu,Min-Goo Lee,Hyo-Jong Kim,Sung-Gil Chi

Nature 557:381-386 PubMed29720660

2018

Catalytic activation of β-arrestin by GPCRs.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsie Eichel,Damien Jullié,Benjamin Barsi-Rhyne,Naomi R Latorraca,Matthieu Masureel,Jean-Baptiste Sibarita,Ron O Dror,Mark von Zastrow
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com