AB277526

Anti-SEPP1 antibody [EPR24174-24]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SEPP1 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SELP, SEPP1, SELENOP, Selenoprotein P, SeP

6 Images

Flow Cytometry (Intracellular) - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SEPP1 with ab277526 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labelling SEPP1 with ab277526 at 1/50 (12.3 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

SEPP1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab277526 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

Lane 2 : ab277526 IP in HepG2 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab277526 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-SEPP1 antibody [EPR24174-24] (ab277526)

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 19345254, 27859223).

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-SEPP1 antibody [EPR24174-24] (ab277526) at 1/1000 dilution

All lanes:

Human plasma at 10 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 19345254, 27859223).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-SEPP1 antibody [EPR24174-24] (ab277526) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) (treated with 300ng/ml Brefeldin A (BFA) for 24 hours) whole cell lysate at 15 µg

Lane 2:

HepG2 (treated with 300ng/ml Brefeldin A (BFA) for 24 hours) whole cell lysate treated with PNGase F at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 35-47 kDa,50-60 kDa

false

Lab

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 19345254, 27859223).

Low expression : Human skeletal muscle(PMID : 23038251).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-SEPP1 antibody [EPR24174-24] (ab277526) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-SEPP1 antibody [EPR24174-24] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24174-24

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPP1 also known as Selenoprotein P is a glycoprotein characterized by multiple selenocysteine residues which play a critical role in its function. SEPP1 has a molecular mass of approximately 60 kDa. It expresses predominantly in the liver but can be found in other tissues like the brain kidney and plasma. In the liver it is involved in selenium transport and homeostasis carrying selenium to other tissues through the blood.

Biological function summary

SEPP1 acts as an antioxidant helping to defend cells against damage from reactive oxygen species. It exists as part of a complex system within the body to balance oxidative stress. Moreover SEPP1 contributes to the recycling of selenium in the body ensuring adequate supply for selenoprotein synthesis in various tissues. It serves essential functions in neurological health and immune response leveraging its selenium content to protect cells and maintain cellular activities.

Pathways

SEPP1 is significantly involved in selenium homeostasis and the oxidative stress response pathways. It interacts with proteins like glutathione peroxidase (GPx) and thioredoxin reductase which also depend on selenium for their antioxidant activities. The involvement of SEPP1 in these pathways is critical for mitigating oxidative damage and supporting the function of other selenium-dependent enzymes essential for maintaining cellular health.

SEPP1 is associated with neurodegenerative diseases and certain types of cancer. Dysregulation of SEPP1 and selenium homeostasis may contribute to the development and progression of conditions such as Alzheimer's disease potentially involving interactions with amyloid precursor protein (APP) and tau proteins. In cancer alterations in SEPP1 expression have been linked to colorectal cancer through pathways intersecting with proteins influencing cell proliferation and apoptosis such as Bcl-2 and p53 indicating its potential role in tumor biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.

See full target information SELENOP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:24 PubMed39972392

2025

KAT8 catalyzes the acetylation of SEPP1 at lysine 247/249 and modulates the activity of CD8 T cells via LRP8 to promote anti-tumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongfei Zhu,Gang Nie,Xiaobo Peng,Xianbao Zhan,Dan Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEPP1 antibody [EPR24174-24]

Flow Cytometry (Intracellular) - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Immunocytochemistry/ Immunofluorescence - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Immunoprecipitation - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Western blot - Anti-SEPP1 antibody [EPR24174-24] (AB277526)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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