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AB236956

Anti-SEPSECS antibody

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(2 Publications)

Rabbit Polyclonal SEPSECS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEPSECS aa 1-500.

View Alternative Names

TRNP48, SEPSECS, O-phosphoseryl-tRNA(Sec) selenium transferase, Liver-pancreas antigen, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Selenocysteinyl-tRNA(Sec) synthase, Sep-tRNA:Sec-tRNA synthase, Soluble liver antigen, UGA suppressor tRNA-associated protein, tRNA(Ser/Sec)-associated antigenic protein, LP, Sec synthase, SepSecS, SLA

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPSECS antibody (AB236956)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPSECS antibody (AB236956)

Paraffin-embedded human kidney tissue stained for SEPSECS with ab236956 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SEPSECS antibody (AB236956)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SEPSECS antibody (AB236956)

PC-3 (Human prostate adenocarcinoma cell line) cells stained for SEPSECS (Green) using ab236956 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-SEPSECS antibody (AB236956)
  • WB

Supplier Data

Western blot - Anti-SEPSECS antibody (AB236956)

All lanes:

Western blot - Anti-SEPSECS antibody (ab236956) at 1/500 dilution

All lanes:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SEPSECS aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

Q9HD40

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SEPSECS protein also known as SEP (O-phosphoseryl-tRNA:selenocysteinyl-tRNA synthase) plays an important role in selenocysteine biosynthesis. It weighs approximately 53 kDa. This enzyme converts phosphoserine-tRNA^Sec to selenocysteinyl-tRNA^Sec facilitating the incorporation of selenocysteine into proteins. SEPSECS is expressed in various tissues throughout the body including liver kidney and brain making it an important target for understanding protein synthesis involving selenium.
Biological function summary

SEPSECS is involved in the unique process of selenoprotein production. It operates as part of a complex that includes multiple other proteins like SPS1 and EEFSEC which assist in the incorporation of the rare amino acid selenocysteine into selenoproteins. These selenoproteins play important roles in antioxidant defense and redox homeostasis impacting cellular health and stress responses. SEPSECS ensures accuracy in this specialized translation process which is essential for proper cell function.

Pathways

Selenocysteine biosynthesis involves SEPSECS interacting with the SECIS element-binding protein which guides the cellular machinery for incorporating selenocysteine at UGA codons in mRNA that normally signal stop in standard genetic code. This process connects SEPSECS with critical pathways such as selenium metabolism and selenoprotein biosynthesis. The pathway also involves interactions with proteins like selenophosphate synthetase and selenocysteine synthase which work in concert to manage selenium's incorporation into proteins.

SEPSECS mutations can lead to complex neurological conditions including pontocerebellar hypoplasia. These disorders are typically characterized by developmental delays and movement issues linked to improper selenoprotein function. Disruptions in SEPSECS activity can also relate to decreased antioxidant capabilities affecting proteins like glutathione peroxidase and thioredoxin reductase which are important for protecting cells from oxidative stress. Understanding SEPSECS aids in identifying potential therapeutic targets in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
See full target information SEPSECS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed39817450

2025

Clonal analysis of SepSecS-specific B and T cells in autoimmune hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Kramer,Federico Mele,Sandra Jovic,Blanca Maria Fernandez,David Jarrossay,Jun Siong Low,Christiane Sokollik,Magdalena Filipowicz Sinnreich,Sylvie Ferrari-Lacraz,Giorgina Mieli-Vergani,Diego Vergani,Antonio Lanzavecchia,Antonino Cassotta,Benedetta Terziroli Beretta-Piccoli,Federica Sallusto

Oncology reports 41:3347-3354 PubMed31002350

2019

Upregulated histamine receptor H3 promotes tumor growth and metastasis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deliang Yu,Jing Zhao,Yuhong Wang,Jing Hu,Qinchuan Zhao,Jibin Li,Jianjun Zhu
View all publications
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Product promise

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