Goat Polyclonal SEPT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEPTIN4 aa 400-450.
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Publications
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- Molecular medicine reports 22:1179-11862020Septin4 regulates endoplasmic reticulum stress and apoptosis in melatonin‑induced osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Tao et. al.PubMed 32626973
- International journal of biological sciences 16:1917-19282020Septin4 promotes cell death in human colon cancer cells by interacting with BAX.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zhao et. al.PubMed 32398959
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SEPTIN4 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link O43236
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster, Dog, Pig, Monkey, Zebrafish, Gorilla, Orangutan, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster, Dog, Pig, Monkey, Zebrafish, Gorilla, Orangutan, Cow | Dilution info - | Notes - |
Target data
Function
Filament-forming cytoskeletal GTPase (Probable). Pro-apoptotic protein involved in LGR5-positive intestinal stem cell and Paneth cell expansion in the intestines, via its interaction with XIAP (By similarity). May also play a role in the regulation of cell fate in the intestine (By similarity). Positive regulator of apoptosis involved in hematopoietic stem cell homeostasis; via its interaction with XIAP (By similarity). Negative regulator of repair and hair follicle regeneration in response to injury, due to inhibition of hair follicle stem cell proliferation, potentially via its interaction with XIAP (By similarity). Plays an important role in male fertility and sperm motility (By similarity). During spermiogenesis, essential for the establishment of the annulus (a fibrous ring structure connecting the midpiece and the principal piece of the sperm flagellum) which is a requisite for the structural and mechanical integrity of the sperm (By similarity). Involved in the migration of cortical neurons and the formation of neuron leading processes during embryonic development (By similarity). Required for dopaminergic metabolism in presynaptic autoreceptors; potentially via activity as a presynaptic scaffold protein (By similarity). Isoform ARTS. Required for the induction of cell death mediated by TGF-beta and possibly by other apoptotic stimuli (PubMed:11146656, PubMed:15837787). Induces apoptosis through binding and inhibition of XIAP resulting in significant reduction in XIAP levels, leading to caspase activation and cell death (PubMed:15029247). Mediates the interaction between BCL2 and XIAP, thereby positively regulating the ubiquitination and degradation of BCL2 and promoting apoptosis (PubMed:29020630).
Alternative names
C17orf47, PNUTL2, SEP4, SEPT4, hucep-7, SEPTIN4, Septin-4, Bradeion beta, Brain protein H5, CE5B3 beta, Cell division control-related protein 2, Peanut-like protein 2, hCDCREL-2
Recommended products
Goat Polyclonal SEPT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SEPTIN4 aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SEPTIN4 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link O43236
- Purification technique
- Affinity purification Immunogen
- Specificity
ab166788 is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1) of Human SEPT4.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEPT4 also known as septin 4 or PC-Tse1 is a member of the septin family of GTP-binding proteins which have roles in cytoskeletal organization. This protein has a molecular mass of approximately 48 kDa and is expressed in various tissues including the brain and testes. SEPT4's mechanical function involves forming hetero-oligomeric complexes that contribute to cellular processes like cytokinesis where it helps in cytokinetic abscission during cell division. Its presence in diverse cell types reflects its role in maintaining cellular architecture and integrity.
- Biological function summary
SEPT4 functions as part of the septin complex which is important for cytoskeletal remodeling and membrane dynamics. It interacts with other septins to form filamentous structures that organize cellular compartments and ensure membrane rigidity and shape. SEPT4 also plays a role in apoptosis particularly in promoting the mitochondrial pathway of apoptotic cell death. Its ability to localize to mitochondria and influence mitochondrial morphology underlines its involvement in cellular stress responses.
- Pathways
SEPT4 engages in significant interactions in the apoptosis and cell cycle pathways. Within the apoptosis pathway SEPT4 influences mitochondrial cytochrome c release indirectly associating with proteins like BCL2 which regulates cell death. In the cell cycle pathway SEPT4's role in cytokinesis highlights its interaction with kinases that govern cell division checkpoints. These pathways emphasize SEPT4's dual role in controlling both cell structure and survival.
- Associated diseases and disorders
SEPT4 has been linked to neurodegenerative diseases and cancer. In Parkinson's disease SEPT4 interacts with proteins like Parkin where it influences the clearance of damaged mitochondrial components suggesting a protective role against neurodegeneration. In the context of cancer aberrations in SEPT4 expression or function can lead to improper cell division potentially contributing to tumorigenesis. Its involvement in such diverse conditions highlights SEPT4's importance in disease pathogenesis and highlights its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SEPT4 antibody (ab166788)
Primary incubation: 1 hour.
All lanes: Western blot - Anti-SEPT4 antibody (ab166788) at 1 µg/mL
All lanes: Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT4 antibody (ab166788)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cerebellum) tissue labeling SEPT4 with ab166788 at 3.75 µg/ml.
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