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AB166788

Anti-SEPT4 antibody

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(5 Publications)

Goat Polyclonal SEPT4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SEPTIN4 aa 400-450.

View Alternative Names

C17orf47, PNUTL2, SEP4, SEPT4, hucep-7, SEPTIN4, Septin-4, Bradeion beta, Brain protein H5, CE5B3 beta, Cell division control-related protein 2, Peanut-like protein 2, hCDCREL-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT4 antibody (AB166788)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT4 antibody (AB166788)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cerebellum) tissue labeling SEPT4 with ab166788 at 3.75 µg/ml.

Western blot - Anti-SEPT4 antibody (AB166788)
  • WB

Unknown

Western blot - Anti-SEPT4 antibody (AB166788)

Primary incubation : 1 hour.

All lanes:

Western blot - Anti-SEPT4 antibody (ab166788) at 1 µg/mL

All lanes:

Human heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 55 kDa

Observed band size: 50 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SEPTIN4 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

O43236

Specificity

ab166788 is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1) of Human SEPT4.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPT4 also known as septin 4 or PC-Tse1 is a member of the septin family of GTP-binding proteins which have roles in cytoskeletal organization. This protein has a molecular mass of approximately 48 kDa and is expressed in various tissues including the brain and testes. SEPT4's mechanical function involves forming hetero-oligomeric complexes that contribute to cellular processes like cytokinesis where it helps in cytokinetic abscission during cell division. Its presence in diverse cell types reflects its role in maintaining cellular architecture and integrity.
Biological function summary

SEPT4 functions as part of the septin complex which is important for cytoskeletal remodeling and membrane dynamics. It interacts with other septins to form filamentous structures that organize cellular compartments and ensure membrane rigidity and shape. SEPT4 also plays a role in apoptosis particularly in promoting the mitochondrial pathway of apoptotic cell death. Its ability to localize to mitochondria and influence mitochondrial morphology underlines its involvement in cellular stress responses.

Pathways

SEPT4 engages in significant interactions in the apoptosis and cell cycle pathways. Within the apoptosis pathway SEPT4 influences mitochondrial cytochrome c release indirectly associating with proteins like BCL2 which regulates cell death. In the cell cycle pathway SEPT4's role in cytokinesis highlights its interaction with kinases that govern cell division checkpoints. These pathways emphasize SEPT4's dual role in controlling both cell structure and survival.

SEPT4 has been linked to neurodegenerative diseases and cancer. In Parkinson's disease SEPT4 interacts with proteins like Parkin where it influences the clearance of damaged mitochondrial components suggesting a protective role against neurodegeneration. In the context of cancer aberrations in SEPT4 expression or function can lead to improper cell division potentially contributing to tumorigenesis. Its involvement in such diverse conditions highlights SEPT4's importance in disease pathogenesis and highlights its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Filament-forming cytoskeletal GTPase (Probable). Pro-apoptotic protein involved in LGR5-positive intestinal stem cell and Paneth cell expansion in the intestines, via its interaction with XIAP (By similarity). May also play a role in the regulation of cell fate in the intestine (By similarity). Positive regulator of apoptosis involved in hematopoietic stem cell homeostasis; via its interaction with XIAP (By similarity). Negative regulator of repair and hair follicle regeneration in response to injury, due to inhibition of hair follicle stem cell proliferation, potentially via its interaction with XIAP (By similarity). Plays an important role in male fertility and sperm motility (By similarity). During spermiogenesis, essential for the establishment of the annulus (a fibrous ring structure connecting the midpiece and the principal piece of the sperm flagellum) which is a requisite for the structural and mechanical integrity of the sperm (By similarity). Involved in the migration of cortical neurons and the formation of neuron leading processes during embryonic development (By similarity). Required for dopaminergic metabolism in presynaptic autoreceptors; potentially via activity as a presynaptic scaffold protein (By similarity).. Isoform ARTS. Required for the induction of cell death mediated by TGF-beta and possibly by other apoptotic stimuli (PubMed : 11146656, PubMed : 15837787). Induces apoptosis through binding and inhibition of XIAP resulting in significant reduction in XIAP levels, leading to caspase activation and cell death (PubMed : 15029247). Mediates the interaction between BCL2 and XIAP, thereby positively regulating the ubiquitination and degradation of BCL2 and promoting apoptosis (PubMed : 29020630).
See full target information SEPTIN4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 37:1629-1641 PubMed35258167

2022

LINC01588 regulates WWP2-mediated cardiomyocyte injury by interacting with HNRNPL.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Song,Xiaoyue Ren,Feng Gao,Fei Li,Jing Zhou,Junmin Chen,Yunqing Zhang

Experimental and therapeutic medicine 22:763 PubMed34035860

2021

Septin 4 activates PPARγ/LXRα signaling by upregulating ABCA1 and ABCG1 expression to inhibit the formation of THP-1 macrophage-derived foam cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Song,Guoliang Yan,Haihui Wang,Danfei Lou

Molecular medicine reports 22:1179-1186 PubMed32626973

2020

Septin4 regulates endoplasmic reticulum stress and apoptosis in melatonin‑induced osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Tao,Sichao Zhao,Zhengbo Tao,Kaicheng Wen,Siming Zhou,Wacili Da,Yue Zhu

International journal of biological sciences 16:1917-1928 PubMed32398959

2020

Septin4 promotes cell death in human colon cancer cells by interacting with BAX.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhao,Hao Feng,Yang Wang,Yanmei Wu,Qiqiang Guo,Yanling Feng,Mengtao Ma,Wendong Guo,Xiaoyu Song,Ying Zhang,Shuai Han,Liu Cao

Developmental cell 44:248-260.e4 PubMed29290587

2018

RAPGEF5 Regulates Nuclear Translocation of β-Catenin.

Applications

Unspecified application

Species

Unspecified reactive species

John N Griffin,Florencia Del Viso,Anna R Duncan,Andrew Robson,Woong Hwang,Saurabh Kulkarni,Karen J Liu,Mustafa K Khokha
View all publications
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