AB154228

Anti-SEPT5 antibody

(1 Review)

(2 Publications)

Rabbit Polyclonal SEPT5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN5 aa 150 to C-terminus.

View Alternative Names

PNUTL1, SEPT5, SEPTIN5, Septin-5, Cell division control-related protein 1, Peanut-like protein 1, CDCrel-1

2 Images

Unknown

Western blot - Anti-SEPT5 antibody (AB154228)

10% SDS PAGE

All lanes:

Western blot - Anti-SEPT5 antibody (ab154228) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 43 kDa

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Unknown

Western blot - Anti-SEPT5 antibody (AB154228)

10% SDS PAGE

All lanes:

Western blot - Anti-SEPT5 antibody (ab154228) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human SEPTIN5 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99719

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPT5 also known as PNUTL1 or hCNTRL is a protein involved in the mechanics of cellular division and membrane dynamics. It has a molecular mass of about 39 kDa and is predominantly found in neuronal and hematopoietic tissues. SEPT5 functions as part of the septin family which plays a role in cytoskeletal rearrangements. Within cells SEPT5 assembles into filaments essential for maintaining structural integrity during cell division.

Biological function summary

SEPT5 is involved in bundling and organizing actin filaments. It forms part of a larger septin complex which includes other septin proteins like SEPT2 and SEPT7. This complex is responsible for the proper organization and stability of cytoskeletal structures which is important for processes like cytokinesis and exocytosis. SEPT5's ability to bind lipid membranes indicates its role in membrane-trafficking processes. Its interactions with vesicular proteins suggest SEPT5's involvement in neurotransmitter release.

Pathways

SEPT5 plays a role in the cell cycle and synaptic vesicle trafficking pathways. In the cell cycle SEPT5 collaborates with proteins such as Cdc42 and calmodulin to ensure accurate division timing and fidelity. In synaptic vesicle trafficking SEPT5 associates with proteins like syntaxin and SNARE complexes contributing to the regulation of neurotransmitter release. These pathways highlight the importance of SEPT5 in both cell proliferation and nervous system functioning.

SEPT5 has been implicated in conditions like Parkinson's disease and acute myeloid leukemia (AML). In Parkinson's disease SEPT5 may interact with alpha-synuclein which is known to disrupt synaptic function and contribute to the pathogenesis of the disorder. In AML deregulation of SEPT5 expression disturbs normal cell division processes. Its interactions with other septins and cell cycle regulators have implications for understanding its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).

See full target information SEPTIN5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 125:e30529 PubMed38308620

2024

Palmitoylation is required for Sept8-204 and Sept5 to form vesicle-like structure and colocalize with synaptophysin.

Applications

Unspecified application

Species

Unspecified reactive species

Huicong Liu,Rong Tan,Jia Tong,Shuo Wen,Can Wu,Muding Rao,Jiangli Zhu,Shiqian Qi,Eryan Kong

Proteomes 5: PubMed28895884

2017

Interaction of Recombinant Gallus gallus SEPT5 and Brain Proteins of H5N1-Avian Influenza Virus-Infected Chickens.

Applications

Species

Unspecified reactive species

Jasmine Elanie Khairat,Vinod Balasubramaniam,Iekhsan Othman,Abdul Rahman Omar,Sharifah Syed Hassan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEPT5 antibody

Western blot - Anti-SEPT5 antibody (AB154228)

Western blot - Anti-SEPT5 antibody (AB154228)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise