AB233431

Anti-SEPT5 antibody

Name: Anti-SEPT5 antibody (ab233431) | Abcam
Brand: Abcam Limited
SKU: AB233431
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SEPT5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN5 aa 50-350.

View Alternative Names

PNUTL1, SEPT5, SEPTIN5, Septin-5, Cell division control-related protein 1, Peanut-like protein 1, CDCrel-1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human stomach tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human brain tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Formalin-fixed, paraffin-embedded human prostate tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-SEPT5 antibody (AB233431)

All lanes:

Western blot - Anti-SEPT5 antibody (ab233431) at 3 µg/mL

All lanes:

Recombinant human SEPT5 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-SEPT5 antibody (AB233431)

All lanes:

Western blot - Anti-SEPT5 antibody (ab233431) at 3 µg/mL

Lane 1:

Pig brain lysate

Lane 2:

Pig heart lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SEPTIN5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q99719

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPT5 also known as PNUTL1 or hCNTRL is a protein involved in the mechanics of cellular division and membrane dynamics. It has a molecular mass of about 39 kDa and is predominantly found in neuronal and hematopoietic tissues. SEPT5 functions as part of the septin family which plays a role in cytoskeletal rearrangements. Within cells SEPT5 assembles into filaments essential for maintaining structural integrity during cell division.

Biological function summary

SEPT5 is involved in bundling and organizing actin filaments. It forms part of a larger septin complex which includes other septin proteins like SEPT2 and SEPT7. This complex is responsible for the proper organization and stability of cytoskeletal structures which is important for processes like cytokinesis and exocytosis. SEPT5's ability to bind lipid membranes indicates its role in membrane-trafficking processes. Its interactions with vesicular proteins suggest SEPT5's involvement in neurotransmitter release.

Pathways

SEPT5 plays a role in the cell cycle and synaptic vesicle trafficking pathways. In the cell cycle SEPT5 collaborates with proteins such as Cdc42 and calmodulin to ensure accurate division timing and fidelity. In synaptic vesicle trafficking SEPT5 associates with proteins like syntaxin and SNARE complexes contributing to the regulation of neurotransmitter release. These pathways highlight the importance of SEPT5 in both cell proliferation and nervous system functioning.

SEPT5 has been implicated in conditions like Parkinson's disease and acute myeloid leukemia (AML). In Parkinson's disease SEPT5 may interact with alpha-synuclein which is known to disrupt synaptic function and contribute to the pathogenesis of the disorder. In AML deregulation of SEPT5 expression disturbs normal cell division processes. Its interactions with other septins and cell cycle regulators have implications for understanding its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).

See full target information SEPTIN5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine 264:9-19 PubMed38839357

2024

Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Lu,Jiabao Ma,Luying Wei,Jing Fu,Xiaoxia Li,Kedao Lai,Xin Li,Bingyu Xia,Bin Bin,Aicun Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEPT5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (AB233431)

Western blot - Anti-SEPT5 antibody (AB233431)

Western blot - Anti-SEPT5 antibody (AB233431)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise