Rabbit Polyclonal SEPT5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN5 aa 50-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SEPTIN5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q99719
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).
Alternative names
PNUTL1, SEPT5, SEPTIN5, Septin-5, Cell division control-related protein 1, Peanut-like protein 1, CDCrel-1
Recommended products
Rabbit Polyclonal SEPT5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN5 aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SEPTIN5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q99719
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEPT5 also known as PNUTL1 or hCNTRL is a protein involved in the mechanics of cellular division and membrane dynamics. It has a molecular mass of about 39 kDa and is predominantly found in neuronal and hematopoietic tissues. SEPT5 functions as part of the septin family which plays a role in cytoskeletal rearrangements. Within cells SEPT5 assembles into filaments essential for maintaining structural integrity during cell division.
- Biological function summary
SEPT5 is involved in bundling and organizing actin filaments. It forms part of a larger septin complex which includes other septin proteins like SEPT2 and SEPT7. This complex is responsible for the proper organization and stability of cytoskeletal structures which is important for processes like cytokinesis and exocytosis. SEPT5's ability to bind lipid membranes indicates its role in membrane-trafficking processes. Its interactions with vesicular proteins suggest SEPT5's involvement in neurotransmitter release.
- Pathways
SEPT5 plays a role in the cell cycle and synaptic vesicle trafficking pathways. In the cell cycle SEPT5 collaborates with proteins such as Cdc42 and calmodulin to ensure accurate division timing and fidelity. In synaptic vesicle trafficking SEPT5 associates with proteins like syntaxin and SNARE complexes contributing to the regulation of neurotransmitter release. These pathways highlight the importance of SEPT5 in both cell proliferation and nervous system functioning.
- Associated diseases and disorders
SEPT5 has been implicated in conditions like Parkinson's disease and acute myeloid leukemia (AML). In Parkinson's disease SEPT5 may interact with alpha-synuclein which is known to disrupt synaptic function and contribute to the pathogenesis of the disorder. In AML deregulation of SEPT5 expression disturbs normal cell division processes. Its interactions with other septins and cell cycle regulators have implications for understanding its role in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Western blot - Anti-SEPT5 antibody (ab233431)
All lanes: Western blot - Anti-SEPT5 antibody (ab233431) at 3 µg/mL
All lanes: Recombinant human SEPT5 protein
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Western blot - Anti-SEPT5 antibody (ab233431)
All lanes: Western blot - Anti-SEPT5 antibody (ab233431) at 3 µg/mL
Lane 1: Pig brain lysate
Lane 2: Pig heart lysate
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human stomach tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human brain tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human prostate tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT5 antibody (ab233431)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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