Rabbit Polyclonal SEPT7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN7 aa 150-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SEPTIN7 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q16181
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830).
Alternative names
CDC10, SEPT7, SEPTIN7, Septin-7, CDC10 protein homolog
Recommended products
Rabbit Polyclonal SEPT7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN7 aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SEPTIN7 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q16181
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEPT7 also known as Septin-7 or CDC10 is a protein that belongs to the septin family. Septins are GTP-binding proteins known for forming hetero-oligomeric complexes. SEPT7 has an approximate molecular mass of 52 kDa. It is expressed in a variety of tissues including the brain heart liver and kidneys among others. Mechanically SEPT7 plays a role in forming filaments and rings serving as scaffolds for other proteins and contributing to the structural organization of the cytoskeleton.
- Biological function summary
In cellular environments SEPT7 is a part of the septin complex which is important for maintaining cell shape and polarity. It participates in processes involving cell division particularly during cytokinesis where it helps in forming the contractile ring necessary for dividing cells. SEPT7 also interacts with actin and microtubules facilitating cell movement and intracellular transport. This protein's dynamics contribute to the stability and segregation of cellular components during cell division.
- Pathways
SEPT7 plays a role in the regulation of the actin cytoskeleton and cell cycle pathways. It interacts with proteins like actin an important component in cellular motion and structure. SEPT7's involvement in the cellular cycle pathway connects it to proteins such as CDC42 which regulate cell proliferation and differentiation. These interactions make SEPT7 significant in controlling the proper progression through different cell cycle phases.
- Associated diseases and disorders
SEPT7 has connections to cancer and neurological disorders. Its disrupted expression has been linked to tumorigenesis as abnormalities in cell division and cytoskeletal organization can promote malignant transformation. In neurological contexts SEPT7 interacts with presynaptic proteins and its dysregulation is related to neurodegenerative diseases such as Alzheimer's disease. Deficiencies in SEPT7 may lead to improper synaptic functioning contributing to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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8 product images
Western blot - Anti-SEPT7 antibody (ab244434)
All lanes: Western blot - Anti-SEPT7 antibody (ab244434) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line9 cell lysate
Lane 2: NBT II (rat cell line) cell lysate
Predicted band size: 51 kDa
Western blot - Anti-SEPT7 antibody (ab244434)
All lanes: Western blot - Anti-SEPT7 antibody (ab244434) at 0.4 µg/mL
All lanes: U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)
Paraffin-embedded human lymph node tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)
Paraffin-embedded human testis tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)
Paraffin-embedded human skeletal muscle tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. No positivity in myocytes, as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)
Paraffin-embedded human cerebral cortex tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)
Paraffin-embedded human fallopian tube tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody (ab244434)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEPT7 (green) using ab244434 at 4 μg/ml in ICC/IF.
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