Rabbit Recombinant Monoclonal SEPT7 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (AB245761), expandable thumbnail](https://content.abcam.com/products/images/sept7-antibody-epr12610-bsa-and-azide-free-ab245761--immunocytochemistry-immunofluorescence-img101348.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (AB245761)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830).
Alternative names
Septin-7, CDC10 protein homolog, SEPT7, CDC10, SEPTIN7
Recommended products
Rabbit Recombinant Monoclonal SEPT7 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Alternative names
Septin-7, CDC10 protein homolog, SEPT7, CDC10, SEPTIN7
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR12610
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab245761 is the carrier-free version of Anti-SEPT7 antibody [EPR12610] ab175229.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEPT7 also known as Septin-7 or CDC10 is a protein that belongs to the septin family. Septins are GTP-binding proteins known for forming hetero-oligomeric complexes. SEPT7 has an approximate molecular mass of 52 kDa. It is expressed in a variety of tissues including the brain heart liver and kidneys among others. Mechanically SEPT7 plays a role in forming filaments and rings serving as scaffolds for other proteins and contributing to the structural organization of the cytoskeleton.
- Biological function summary
In cellular environments SEPT7 is a part of the septin complex which is important for maintaining cell shape and polarity. It participates in processes involving cell division particularly during cytokinesis where it helps in forming the contractile ring necessary for dividing cells. SEPT7 also interacts with actin and microtubules facilitating cell movement and intracellular transport. This protein's dynamics contribute to the stability and segregation of cellular components during cell division.
- Pathways
SEPT7 plays a role in the regulation of the actin cytoskeleton and cell cycle pathways. It interacts with proteins like actin an important component in cellular motion and structure. SEPT7's involvement in the cellular cycle pathway connects it to proteins such as CDC42 which regulate cell proliferation and differentiation. These interactions make SEPT7 significant in controlling the proper progression through different cell cycle phases.
- Associated diseases and disorders
SEPT7 has connections to cancer and neurological disorders. Its disrupted expression has been linked to tumorigenesis as abnormalities in cell division and cytoskeletal organization can promote malignant transformation. In neurological contexts SEPT7 interacts with presynaptic proteins and its dysregulation is related to neurodegenerative diseases such as Alzheimer's disease. Deficiencies in SEPT7 may lead to improper synaptic functioning contributing to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (ab245761), expandable thumbnail](https://content.abcam.com/products/images/sept7-antibody-epr12610-bsa-and-azide-free-ab245761--immunocytochemistry-immunofluorescence-img101348.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (ab245761)")
Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] - BSA and Azide free (ab245761)
Immunofluorescent analysis of HeLa cells labeling SEPT7 with Anti-SEPT7 antibody [EPR12610] ab175229 at 1/50 dilution. DAPI nuclear staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (Anti-SEPT7 antibody [EPR12610] ab175229).
Downloads
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