AB183529

Anti-Septin 11 antibody

Name: Anti-Septin 11 antibody (ab183529) | Abcam
Brand: Abcam Limited
SKU: AB183529
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Septin 11 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SEPTIN11 aa 1-50.

View Alternative Names

SEPT11, SEPTIN11, Septin-11

2 Images

Supplier Data

Immunoprecipitation - Anti-Septin 11 antibody (AB183529)

Detection of Septin 11 by Western Blot of Immunoprecipitate.
ab183529 at 0.2μg/ml labeling Septin 11 in HeLa whole cell lysate immunoprecipitated using ab183529 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Septin 11 antibody (ab183529)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-Septin 11 antibody (AB183529)

All lanes:

Western blot - Anti-Septin 11 antibody (ab183529) at 0.2 µg

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

mouse TCMK1 whole cell lysate at 50 µg

Lane 5:

mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SEPTIN11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NVA2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab183529 was affinity purified using an epitope specific to Septin 11 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Septin 11 also known as SEPT11 functions as a GTPase involved in the formation of filamentous structures. This protein has a molecular mass of approximately 49 kDa. It is expressed in a wide range of tissues but most abundantly in the brain. Septin 11 belongs to the septin family which consists of cytoskeletal proteins important for cellular processes like cytokinesis and vesicle trafficking.

Biological function summary

The protein plays a role in cellular structure and integrity forming a complex with other septins to maintain cellular shape and facilitate mitotic processes. Septin 11 participates in organizing the actin cytoskeleton which affects cell division and movement. Its interaction with microtubules also suggests roles in intracellular transport and neural communication.

Pathways

Septin 11 is involved in actin and microtubule dynamics. It interacts with pathways that govern cellular cycle regulation and signal transduction. Related proteins like Septin 2 and Septin 9 play interactive roles in these pathways contributing to the complex orchestration of cellular organization and division. Their coordinated function ensures proper cell cycle progression and developmental processes.

Abnormalities in Septin 11 expression or function link to neurodegenerative diseases and cancer. Dysregulation can contribute to conditions such as Alzheimer's disease potentially through its interactions with tau proteins. Additionally altered Septin 11 expression associates with tumorigenesis as seen in some forms of leukemia where it interacts with proteins like BCR-ABL. Understanding Septin 11’s role in these diseases aids in the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.

See full target information SEPTIN11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4101 PubMed37491347

2023

Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Yu,Lin Xu,Haiyan Chu,Jun Peng,Anastasia Sacharidou,Hsi-Hsien Hsieh,Ada Weinstock,Sohaib Khan,Liqian Ma,José Gabriel Barcia Durán,Jeffrey McDonald,Erik R Nelson,Sunghee Park,Donald P McDonnell,Kathryn J Moore,Lily Jun-Shen Huang,Edward A Fisher,Chieko Mineo,Linzhang Huang,Philip W Shaul

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Septin 11 antibody

Immunoprecipitation - Anti-Septin 11 antibody (AB183529)

Western blot - Anti-Septin 11 antibody (AB183529)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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