Rabbit Polyclonal Septin 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN2 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Cow | Predicted | Predicted | Predicted |
Orangutan | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Select an associated product type
Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
DIFF6, KIAA0158, NEDD5, SEPT2, SEPT2, NEDD5, KIAA0158, DIFF6, SEPTIN2, Septin-2, Neural precursor cell expressed developmentally down-regulated protein 5, NEDD-5
Rabbit Polyclonal Septin 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN2 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Septin 2 also known as septin-2 or SEPT2 is a GTP-binding protein with a molecular mass of approximately 41 kDa. It functions mechanically as a major component of the cytoskeletal structure playing an essential role in cellular organization and division. Septin 2 is expressed across various human tissues and cell types indicative of its fundamental role in maintaining cellular integrity. It participates in organizing the actin cytoskeleton and is necessary for cytokinesis.
Septin 2 integrates into a filamentous network interacting closely with other septins to form hetero-oligomeric complexes essential for cellular dynamics. The protein contributes to the formation of diffusion barriers within membranes facilitating compartmentalization in the cells. As a structural element it supports cellular morphogenesis polarity and vesicle trafficking. The collaborative action within septin complexes ensures effective cell cycle progression and signal transduction.
Septin 2 plays a part in key cellular pathways including the cell cycle and apoptosis pathways. The interaction of Septin 2 with proteins like Cdc42 and Rac1 impacts signaling cascades pivotal for actin cytoskeleton organization. The septin complex is important for modulating these pathways as it influences cellular responses to environmental cues. Through these pathways Septin 2 contributes to critical processes such as mitotic spindle positioning and stability.
Altered expression or function of Septin 2 is linked to cancer including gliomas where septin dysregulation affects tumor growth and metastasis. Additionally the protein has connections to neurodegenerative disorders such as Alzheimer's disease. Within these contexts proteins like Cdc42 and Rac1 further influence Septin 2's role modulating disease progression through pathways involved in cell migration and proliferation. Understanding Septin 2 in these disorders provides insight into potential therapeutic targets for intervention.
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Paraffin-embedded human endometrium stained for Septin2 using ab224410 at 1/200 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in glandular cells.
Paraffin-embedded human small intestine stained for Septin2 using ab224410 at 1/200 dilution in immunohistochemical analysis. Shows weak cytoplasmic positivity in glandular cells.
Paraffin-embedded human cerebral cortex stained for Septin2 using ab224410 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in glial cells.
Paraffin-embedded human testis stained for Septin2 using ab224410 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.
All lanes: Western blot - Anti-Septin 2 antibody (ab224410) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Predicted band size: 41 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Septin 2 (green) using ab224410 at 4 μg/ml in ICC/IF.
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