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Rabbit Polyclonal Septin 8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Septin-8 aa 150 to C-terminus.

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Images

Western blot - Anti-Septin 8 antibody (AB154112), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Septin-8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92599

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cow
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (PubMed:15116257). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).Isoform 4Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.

Alternative names

Recommended products

Rabbit Polyclonal Septin 8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Septin-8 aa 150 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Septin-8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92599
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Septin 8 often referred to as SEPT8 belongs to the septin family of GTPase proteins. It participates in filament formation by polymerizing with other septins. This protein has a molecular weight of around 52 kDa. SEPT8 is expressed in various tissues with significant presence in the brain and heart. Its function depends on cell type and developmental stage exhibiting dynamic patterns of expression which make it integral to cellular processes.

Biological function summary

The protein is involved in maintaining cell structure and polarity. SEPT8 is part of larger septin complexes necessary for correct cytokinesis. It regulates cell division by contributing to the formation of cleavage furrow during mitosis. SEPT8 also influences membrane dynamics and vesicle trafficking showcasing a role in transport within cells.

Pathways

Septin 8 contributes to cytoskeleton organization and vesicle trafficking pathways. It associates with proteins such as actin and myosin playing roles in cell shape changes and intracellular transport. SEPT8 functions alongside other septins in the septin ring structures which are essential for several processes including cell cycle progression and vesicle sorting.

Associated diseases and disorders

Septin 8 has been linked to neurodegenerative conditions and cancer. Abnormal SEPT8 expression or mutations might lead to Alzheimer's disease due to its impact on neuronal transport systems. SEPT8 is also observed in tumorigenesis interfering with normal cell cycle control. In cancer its interaction with other septins could disrupt normal cell proliferation suggesting it as a potential therapeutic target.

Product promise

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2 product images

  • Western blot - Anti-Septin 8 antibody (ab154112), expandable thumbnail

    Western blot - Anti-Septin 8 antibody (ab154112)

    12% SDS PAGE

    All lanes: Western blot - Anti-Septin 8 antibody (ab154112) at 1/2000 dilution

    All lanes: HCT116 whole cell lysate at 30 µg

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
    Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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