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AB191404

Anti-Septin 8 antibody [EPR16099]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal Septin 8 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

KIAA0202, SEPT8, SEPTIN8, Septin-8

8 Images
Flow Cytometry (Intracellular) - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Septin 8 antibody [EPR16099] (AB191404)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Septin 8 with ab191404 at 1/150 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti-rabbit IgG (FITC) was used as secondary antibodyat 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (AB191404)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Septin 8 with ab191404 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • IP

Supplier Data

Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] (AB191404)

Western blot analysis of Septin 8 in K562 cell lysate immunoprecipitated using ab191404 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of K562 lysates.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] (ab191404)

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody [EPR16099] (AB191404)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized C6 cells labeling Septin 8 with ab191404 at 1/200 dilution followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

The two negative controls used anti-Septin 8 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (AB191404)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Septin 8 with ab191404 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • WB

Supplier Data

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • WB

Supplier Data

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

Lane 1:

HCT-116 cell lysate at 20 µg

Lane 2:

U-87 MG cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)
  • WB

Supplier Data

Western blot - Anti-Septin 8 antibody [EPR16099] (AB191404)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

  • Carrier free

    Anti-Septin 8 antibody [EPR16099] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16099

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Septin 8 often referred to as SEPT8 belongs to the septin family of GTPase proteins. It participates in filament formation by polymerizing with other septins. This protein has a molecular weight of around 52 kDa. SEPT8 is expressed in various tissues with significant presence in the brain and heart. Its function depends on cell type and developmental stage exhibiting dynamic patterns of expression which make it integral to cellular processes.
Biological function summary

The protein is involved in maintaining cell structure and polarity. SEPT8 is part of larger septin complexes necessary for correct cytokinesis. It regulates cell division by contributing to the formation of cleavage furrow during mitosis. SEPT8 also influences membrane dynamics and vesicle trafficking showcasing a role in transport within cells.

Pathways

Septin 8 contributes to cytoskeleton organization and vesicle trafficking pathways. It associates with proteins such as actin and myosin playing roles in cell shape changes and intracellular transport. SEPT8 functions alongside other septins in the septin ring structures which are essential for several processes including cell cycle progression and vesicle sorting.

Septin 8 has been linked to neurodegenerative conditions and cancer. Abnormal SEPT8 expression or mutations might lead to Alzheimer's disease due to its impact on neuronal transport systems. SEPT8 is also observed in tumorigenesis interfering with normal cell cycle control. In cancer its interaction with other septins could disrupt normal cell proliferation suggesting it as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (PubMed : 15116257). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).. Isoform 4. Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.
See full target information SEPTIN8
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 13: PubMed40872920

2025

The Immunoproteasome Is Expressed but Dispensable for a Leukemia Infected Cell Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Delphine Béland,Victor Mullins-Dansereau,Karen Geoffroy,Mélissa Viens,Kim Leclerc Desaulniers,Marie-Claude Bourgeois-Daigneault

Cell reports. Medicine 5:101679 PubMed39168102

2024

Proteomic landscape profiling of primary prostate cancer reveals a 16-protein panel for prognosis prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Sun,Jun A,Haolan Yu,Yan Wang,Miaoxia He,Lingling Tan,Honghan Cheng,Jili Zhang,Yingrui Wang,Xiaochen Sun,Mengge Lyu,Min Qu,Lingling Huang,Zijian Li,Wenhui Zhang,Kunpeng Ma,Zhenyang Dong,Weigang Ge,Yun Zhang,Xuan Ding,Bo Yang,Jianguo Hou,Chuanliang Xu,Linhui Wang,Yi Zhu,Tiannan Guo,Xu Gao,Chenghua Yang

Journal of cellular biochemistry 125:e30529 PubMed38308620

2024

Palmitoylation is required for Sept8-204 and Sept5 to form vesicle-like structure and colocalize with synaptophysin.

Applications

Unspecified application

Species

Unspecified reactive species

Huicong Liu,Rong Tan,Jia Tong,Shuo Wen,Can Wu,Muding Rao,Jiangli Zhu,Shiqian Qi,Eryan Kong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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