AB233647

Anti-SERCA1 ATPase antibody [3D1H6]

Name: Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) | Abcam
Brand: Abcam Limited
SKU: AB233647
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal SERCA1 ATPase antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, African green monkey, Recombinant full length protein - Human, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2A1 aa 450-650.

View Alternative Names

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1

4 Images

Flow Cytometry - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab233647 (green) at 1/200 dilution compared with a negetive control (red).

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.

Lane 2:

SERCA1 ATPase (aa487-631)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.

Predicted band size: 110 kDa

false

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution

Lane 1:

C2C12 (mouse myoblast cell line) cell lysate.

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate.

Lane 4:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate.

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate.

Predicted band size: 110 kDa

false

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Expected MW is 42 kDa.

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution

All lanes:

Recombinant human SERCA1 ATPase (aa 487-631) protein.

Predicted band size: 104 kDa,109 kDa,110 kDa,33 kDa,44 kDa,99 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D1H6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human, African green monkey

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ATP2A1 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.

O14983

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SERCA1 ATPase also known as sarco/endoplasmic reticulum Ca2+ ATPase 1 is an important enzyme responsible for the active transport of Ca2+ ions from the cytosol into the sarcoplasmic reticulum which is important for muscle relaxation. This protein has a molecular weight of about 110 kDa. SERCA1 ATPase predominantly expresses in fast-twitch skeletal muscle allowing these muscles to relax rapidly after contraction. The enzyme utilizes ATP to pump calcium ions which highlights its role as an ATPase protein and its functionality in maintaining calcium homeostasis.

Biological function summary

SERCA1 ATPase ensures proper calcium regulation and muscle function by facilitating the reuptake of Ca2+ ions into the sarcoplasmic reticulum following muscle contraction. It does not operate as part of a complex but plays a significant role in calcium ion translocation thereby regulating muscle contraction-relaxation cycles. This ATPase protein is directly involved in muscle physiology and its efficient function is critical for fast muscle fibers.

Pathways

SERCA1 ATPase is a significant component of the calcium signaling and muscle contraction pathways. In the context of muscle contraction the release and reuptake of Ca2+ ions regulated by SERCA1 ATPase are central events. The protein works closely with the ryanodine receptor (RyR) and calsequestrin which also participate in the modulation of intracellular calcium levels. Their interactions ensure precise coordination during muscle contraction and relaxation processes.

Mutations or dysregulation of SERCA1 ATPase can lead to conditions such as Brody disease and certain forms of myopathy. Brody disease is characterized by impaired muscle relaxation which directly relates to the malfunctioning of this Ca2+ ATPase. Additionally the disrupted function of SERCA1 ATPase may also involve interactions with other proteins like the ryanodine receptor which can exacerbate muscle-related symptoms and contribute to the pathophysiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed : 10914677).

See full target information ATP2A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23237 PubMed36193555

2022

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanli Zhang,Yan Feng,Min Wang,Xin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SERCA1 ATPase antibody [3D1H6]

Flow Cytometry - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (AB233647)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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