Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)

Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for mIHC, WB, IHC-P, IP and reacts with Mouse, Human, Rat, Transfected cell lysate - Human samples.

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Images

Multiplex immunohistochemistry - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	WB	IHC-P	IP	ICC/IF	Flow Cyt (Intra)
Human	Expected	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Expected	Not recommended	Not recommended
Rat	Expected	Tested	Tested	Expected	Not recommended	Not recommended
Transfected cell lysate - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information ATP2A1

Function

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).

Alternative names

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1

Recommended products

Rabbit Recombinant Monoclonal SERCA1 ATPase antibody. Carrier free. Suitable for mIHC, WB, IHC-P, IP and reacts with Mouse, Human, Rat, Transfected cell lysate - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28061-7
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314661 is the carrier-free version of Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SERCA1 ATPase also known as sarco/endoplasmic reticulum Ca2+ ATPase 1 is an important enzyme responsible for the active transport of Ca2+ ions from the cytosol into the sarcoplasmic reticulum which is important for muscle relaxation. This protein has a molecular weight of about 110 kDa. SERCA1 ATPase predominantly expresses in fast-twitch skeletal muscle allowing these muscles to relax rapidly after contraction. The enzyme utilizes ATP to pump calcium ions which highlights its role as an ATPase protein and its functionality in maintaining calcium homeostasis.

Biological function summary

SERCA1 ATPase ensures proper calcium regulation and muscle function by facilitating the reuptake of Ca2+ ions into the sarcoplasmic reticulum following muscle contraction. It does not operate as part of a complex but plays a significant role in calcium ion translocation thereby regulating muscle contraction-relaxation cycles. This ATPase protein is directly involved in muscle physiology and its efficient function is critical for fast muscle fibers.

Pathways

SERCA1 ATPase is a significant component of the calcium signaling and muscle contraction pathways. In the context of muscle contraction the release and reuptake of Ca2+ ions regulated by SERCA1 ATPase are central events. The protein works closely with the ryanodine receptor (RyR) and calsequestrin which also participate in the modulation of intracellular calcium levels. Their interactions ensure precise coordination during muscle contraction and relaxation processes.

Associated diseases and disorders

Mutations or dysregulation of SERCA1 ATPase can lead to conditions such as Brody disease and certain forms of myopathy. Brody disease is characterized by impaired muscle relaxation which directly relates to the malfunctioning of this Ca2+ ATPase. Additionally the disrupted function of SERCA1 ATPase may also involve interactions with other proteins like the ryanodine receptor which can exacerbate muscle-related symptoms and contribute to the pathophysiology of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Multiplex immunohistochemistry - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at a 1/5000 (0.103 ug/ml) dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 ug/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 ug/ml) dilution.
Panel A: merged staining of anti-SERCA1 ATPase (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B: anti-SERCA1 ATPase staining cytoplasm in mouse skeletal muscle.
Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human ATP2A2 and ATP2A3.

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human ATP2A1 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human ATP2A2 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human ATP2A3 expression vector containing a His-tag, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 100 kDa, 36 kDa
Exposure time: 10s
Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The lane 2 was developed using a high sensitivity ECL substrate.

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660) at 1/1000 dilution
Lane 1: RD (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblasts) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 100 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: heart, cerebellum.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 36714847).

Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-3: 10 seconds; lanes 4-8: 3 seconds
All lanes: Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human heart tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
Lane 5: Mouse cerebellum tissue lysate at 20 µg
Lane 6: Mouse heart tissue lysate at 20 µg
Lane 7: Rat skeletal muscle tissue lysate at 20 µg
Lane 8: Rat heart tissue lysate at 20 µg
Secondary
Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 4 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa, 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat cardiac muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse cardiac muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human cardiac muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat skeletal muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skeletal muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA1 ATPase with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skeletal muscle. The section was incubated with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (ab314661)
This data was developed using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660, the same antibody clone in a different buffer formulation.
SERCA1 ATPase was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 IP in Human skeletal muscle tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660 in human skeletal muscle tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-SERCA1 ATPase antibody [EPR28061-7] (Anti-SERCA1 ATPase antibody [EPR28061-7] ab314660) at 1/30 dilution
All lanes: Human skeletal muscle tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s