Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA1 ATPase with ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skeletal muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling SERCA1 ATPase with ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human cardiac muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. SERCA1 ATPase was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab314660 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314660 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human skeletal muscle tissue lysate Lane 2 : ab314660 IP in Human skeletal muscle tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314660 in human skeletal muscle tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SERCA1 ATPase antibody [EPR28061-7] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr28061-7-ab314660'>ab314660</a>) at 1/30 dilution

All lanes:

Human skeletal muscle tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat skeletal muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining SERCA1 ATPase with ab314660 at a 1/5000 (0.103 ug/ml) dilution, A2BP1 with ab254413 at 1/2000 (0.26 ug/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 ug/ml) dilution.

Panel A : merged staining of anti-SERCA1 ATPase (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-SERCA1 ATPase staining cytoplasm in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314660, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling SERCA1 ATPase with ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse cardiac muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA1 ATPase with ab314660 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skeletal muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling SERCA1 ATPase with ab314660 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat cardiac muscle. The section was incubated with ab314660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The lane 2 was developed using a high sensitivity ECL substrate. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr28061-7-ab314660'>ab314660</a>) at 1/1000 dilution

Lane 1:

RD (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

Lane 2:

C2C12 (mouse myoblasts) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : heart, cerebellum. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 36714847). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lanes 1-3 : 10 seconds; lanes 4-8 : 3 seconds

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr28061-7-ab314660'>ab314660</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse cerebellum tissue lysate at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Lane 7:

Rat skeletal muscle tissue lysate at 20 µg

Lane 8:

Rat heart tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 4 - 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] - BSA and Azide free (AB314661)

This data was developed using ab314660, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human ATP2A2 and ATP2A3. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [EPR28061-7] (<a href='/en-us/products/primary-antibodies/serca1-atpase-antibody-epr28061-7-ab314660'>ab314660</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ATP2A1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ATP2A2 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human ATP2A3 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 10s